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Ann Biomed Eng. 2017 Dec;45(12):2888-2898. doi: 10.1007/s10439-017-1925-2. Epub 2017 Sep 18.

Development of a High-Throughput Magnetic Separation Device for Malaria-Infected Erythrocytes.

Author information

1
Department of Biomedical Engineering, Carnegie Mellon University, Scott Hall 4N201, 5000 Forbes Avenue, Pittsburgh, PA, 15213, USA. andrea.blue.martin@gmail.com.
2
Department of Biomedical Engineering, Carnegie Mellon University, Scott Hall 4N201, 5000 Forbes Avenue, Pittsburgh, PA, 15213, USA.
3
McGowan Institute for Regenerative Medicine, University of Pittsburgh, 450 Technology Drive, Pittsburgh, PA, 15219, USA.

Abstract

This study describes a non-dilutive high-gradient magnetic separation (HGMS) device intended to continuously remove malaria-infected red blood cells (iRBCs) from the circulation. A mesoscale prototype device with disposable photo-etched ferromagnetic grid and reusable permanent magnet was designed with a computationally-optimized magnetic force. The prototype device was evaluated in vitro using a non-pathogenic analog for malaria-infected blood, comprised of 24% healthy RBCs, 6% human methemoglobin RBCs (metRBCs), and 70% phosphate buffer solution (PBS). The device provided a 27.0 ± 2.2% reduction of metRBCs in a single pass at a flow rate of 77 μL min-1. This represents a clearance rate over 380 times greater throughput than microfluidic devices reported previously. These positive results encourage development of a clinical scale system that would economize time and donor blood for treating severe malaria.

KEYWORDS:

Apheresis; Magnetics; Malaria; Microfluidic; Modeling; Separation; Treatment

PMID:
28924724
PMCID:
PMC5693708
DOI:
10.1007/s10439-017-1925-2
[Indexed for MEDLINE]
Free PMC Article

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