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Methods Mol Biol. 2017;1663:231-252. doi: 10.1007/978-1-4939-7265-4_19.

In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT.

Beliveau BJ1,2,3, Boettiger AN4,5,6, Nir G1, Bintu B4,7, Yin P8,9, Zhuang X10,11,12, Wu CT13.

Author information

1
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
2
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
3
Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
4
Howard Hughes Medical Institute, Cambridge, MA, 02138, USA.
5
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, USA.
6
Department of Developmental Biology, Stanford University, Stanford, CA, 94305, USA.
7
Department of Physics, Harvard University, Cambridge, MA, 02138, USA.
8
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA. py@hms.harvard.edu.
9
Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA. py@hms.harvard.edu.
10
Howard Hughes Medical Institute, Cambridge, MA, 02138, USA. zhuang@chemistry.harvard.edu.
11
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, USA. zhuang@chemistry.harvard.edu.
12
Department of Physics, Harvard University, Cambridge, MA, 02138, USA. zhuang@chemistry.harvard.edu.
13
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA. twu@genetics.med.harvard.edu.

Abstract

OligoSTORM and OligoDNA-PAINT meld the Oligopaint technology for fluorescent in situ hybridization (FISH) with, respectively, Stochastic Optical Reconstruction Microscopy (STORM) and DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) to enable in situ single-molecule super-resolution imaging of nucleic acids. Both strategies enable ≤20 nm resolution and are appropriate for imaging nanoscale features of the genomes of a wide range of species, including human, mouse, and fruit fly (Drosophila).

KEYWORDS:

Chromatin; Chromosomes; DNA-PAINT; FISH; Genome; OligoDNA-PAINT; OligoSTORM; Oligopaint; STORM; Single-molecule; Super-resolution

PMID:
28924672
PMCID:
PMC5919218
DOI:
10.1007/978-1-4939-7265-4_19
[Indexed for MEDLINE]
Free PMC Article

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