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Asian-Australas J Anim Sci. 2018 Mar;31(3):344-353. doi: 10.5713/ajas.17.0441. Epub 2017 Sep 18.

Functional role of Forskolin and PD166285 in the development of denuded mouse oocytes.

Author information

1
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
2
Anhui Provincial Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, Hefei 230036, China.

Abstract

OBJECTIVE:

cAMP and mature promoting factor (MPF) play critical roles during the maturation of mammalian oocytes. The aim of this study was to produce the offspring from denuded oocytes (DOs) in mice by regulating cAMP and MPF.

METHODS:

In this study, we used DOs at the germinal vesicle (GV) stage in mice and regulated levels of cAMP and MPF in DOs by adding Forskolin and PD166285 during in vitro maturation without follicle stimulating hormone and luteinizing hormone, respectively.

RESULTS:

Combined use of 50 μM Forskolin for 3 h and 2.5 μM PD166285 for additional 21 h enhanced the developmental competence of DOs, maturation rate of DOs was 76.71%± 4.11%, blastocyst rate was 18.33%±4.44% after parthenogenetic activation (PA). The DOs could successfully be fertilized with sperm in vitro, cleavage rate was 17.02%±5.82% and blastocyst rate was 5.65%±3.10%. Besides, 2-cell in vitro fertilization embryos from DOs produced 4 normal live offspring (4/34).

CONCLUSION:

The results confirmed that the combination of Forskolin and PD166285 can induce DOs to complete meiosis process and produce normal offspring.

KEYWORDS:

Denuded Oocytes; Forskolin; In vitro Fertilization; Live Offspring; PD166285

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