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Mol Cell. 2017 Oct 5;68(1):130-143.e5. doi: 10.1016/j.molcel.2017.08.016. Epub 2017 Sep 14.

SAGA Is a General Cofactor for RNA Polymerase II Transcription.

Author information

1
Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch, France; Université de Strasbourg, 67404 Illkirch, France.
2
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
3
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA; Université Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France.
4
Molecular Cancer Research and Stem Cell Section, Regenerative Medicine Center and Center for Molecular Medicine, University Medical Center Utrecht c/o Hubrecht Institute, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands.
5
Molecular Cancer Research and Stem Cell Section, Regenerative Medicine Center and Center for Molecular Medicine, University Medical Center Utrecht c/o Hubrecht Institute, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands; German Cancer Consortium (DKTK) partner site Freiburg, German Cancer Research Center (DKFZ) and Department of Urology, Medical Center-University of Freiburg, 79106 Freiburg, Germany.
6
Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch, France; Université de Strasbourg, 67404 Illkirch, France. Electronic address: devys@igbmc.fr.
7
Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch, France; Université de Strasbourg, 67404 Illkirch, France. Electronic address: laszlo@igbmc.fr.

Abstract

Prior studies suggested that SAGA and TFIID are alternative factors that promote RNA polymerase II transcription, with about 10% of genes in S. cerevisiae dependent on SAGA. We reassessed the role of SAGA by mapping its genome-wide location and role in global transcription in budding yeast. We find that SAGA maps to the UAS elements of most genes, overlapping with Mediator binding and irrespective of previous designations of SAGA- or TFIID-dominated genes. Disruption of SAGA through mutation or rapid subunit depletion reduces transcription from nearly all genes, measured by newly synthesized RNA. We also find that the acetyltransferase Gcn5 synergizes with Spt3 to promote global transcription and that Spt3 functions to stimulate TBP recruitment at all tested genes. Our data demonstrate that SAGA acts as a general cofactor required for essentially all RNA polymerase II transcription and is not consistent with the previous classification of SAGA- and TFIID-dominated genes.

KEYWORDS:

RNA polymerase II; SAGA complex; TATA box; TFIID complex; coactivator; transcription initiation

PMID:
28918903
PMCID:
PMC5632562
DOI:
10.1016/j.molcel.2017.08.016
[Indexed for MEDLINE]
Free PMC Article

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