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Carbohydr Polym. 2017 Nov 1;175:170-177. doi: 10.1016/j.carbpol.2017.07.058. Epub 2017 Jul 25.

Preparation and biological activity studies of resveratrol loaded ionically cross-linked chitosan-TPP nanoparticles.

Author information

1
Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Xiongchu Avenue, Wuhan 430073, PR China.
2
Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Xiongchu Avenue, Wuhan 430073, PR China. Electronic address: zhonglu@wit.edu.cn.

Abstract

Nanoparticles with size range of 10-500nm can be efficiently delivered into cancer cells by the Enhanced Permeability and Retention (EPR) effect. Here, we prepared resveratrol (Res) loaded chitosan (CS) nanoparticles with the size of 172-217nm by an ionic cross-linking method, with sodium tripolyphosphate (TPP) as the cross-linking agent, to improve the stability, solubility and tumors targeting of the natural anti-cancer drug Res. The prepared Res loaded CS-TPP nanoparticles presented long-term storage stability and UV light stability. The cumulative drug release from nanoparticles in mimetic tumor tissue condition (pH 6.5) was higher than that in physiological condition (pH 7.4). Further, Res-loaded CS-TPP nanoparticles maintained the antioxidant activity of Res even after UV light irradiation. Cell viability study shows that the as prepared drug loaded nanoparticles had similar antiproliferative activity on hepatocellular carcinoma cells SMMC 7721 and lower cytotoxicity on normal hepatocyte cells L02 compared with free Res. Fluorescence microscopy observation revealed that the nanoparticles were efficiently taken in by SMMC 7721 cells. This work indicates the potential use of drug loaded CS-TPP nanoparticles for the efficient delivery of bioactive Res for chemotherapy.

KEYWORDS:

2, 2-Diphenyl-1-(2, 4, 6-trinitrophenyl) hydrazyl (PubChem CID:294769555); 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (PubChem CID:64965); Acetic acid (PubChem CID: 176); Antioxidant activity; Chitosan; Chitosan (PubChem CID: 21896651); Cytotoxicity; Fluorescein isothiocyanate (PubChem CID: 18730); Hydroxyl ethyl piperazine ethane sulfonic acid (PubChem CID: 23831); Ionic crosslinking; Poloxamer188 (PubChem CID: 24751); Resveratrol; Resveratrol (PubChem CID: 445154); Sodium hydroxide (PubChem CID: 14798); Sodium tripolyphosphate (PubChem CID: 24455)

PMID:
28917853
DOI:
10.1016/j.carbpol.2017.07.058
[Indexed for MEDLINE]

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