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Vaccine. 2017 Oct 9;35(42):5531-5534. doi: 10.1016/j.vaccine.2017.06.021. Epub 2017 Sep 13.

Identification of IBV QX vaccine markers : Should vaccine acceptance by authorities require similar identifications for all live IBV vaccines?

Author information

1
Department of Infection Biology, University of Liverpool, Leahurst Campus, Neston, Cheshire CH64 7TE, United Kingdom.
2
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, 40064 Ozzano dell'Emilia, BO, Italy.
3
Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università, 16, 35020 Legnaro, PD, Italy.
4
Department of Infection Biology, University of Liverpool, Leahurst Campus, Neston, Cheshire CH64 7TE, United Kingdom. Electronic address: cnaylor@liv.ac.uk.

Abstract

IBV genotype QX causes sufficient disease in Europe for several commercial companies to have started developing live attenuated vaccines. Here, one of those vaccines (L1148) was fully consensus sequenced alongside its progenitor field strain (1148-A) to determine vaccine markers, thereby enabling detection on farms. Twenty-eight single nucleotide substitutions were associated with the 1148-A attenuation, of which any combination can identify vaccine L1148 in the field. Sixteen substitutions resulted in amino acid coding changes of which half were in spike. One change in the 1b gene altered the normally highly conserved final 5 nucleotides of the transcription regulatory sequence of the S gene, common to all IBV QX genes. No mutations can currently be associated with the attenuation process. Field vaccination strategies would greatly benefit by such comparative sequence data being mandatorily submitted to regulators prior to vaccine release following a successful registration process.

KEYWORDS:

Farm vaccine detection; IBV; Vaccine registration; Vaccine regulation

PMID:
28917538
DOI:
10.1016/j.vaccine.2017.06.021
[Indexed for MEDLINE]

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