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Anal Chim Acta. 2017 Oct 2;988:58-65. doi: 10.1016/j.aca.2017.07.053. Epub 2017 Aug 11.

Highly selective and sensitive visualization and identification of glycoproteins using multi-functionalized soluble dendrimer.

Author information

1
Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, China.
2
Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, China; Department of Biochemistry, Purdue University, West Lafayette, IN, 47907, USA. Electronic address: zhiani@126.com.
3
Dalian Medical University, Lvshun South Road No 9, Dalian, 116044, China. Electronic address: maxc1978@163.com.

Abstract

Glycoproteins are the most important and complex group of posttranslational modifications known in proteins. Many clinical biomarkers and therapeutic targets in cancer are glycoproteins. However, the isolation of glyco-specific antibodies and their poor stability remains a significant challenge in analytical method and diagnostic development. In this work, for the first time, we present a technology for highly efficient and selective glycosylation analysis on membrane without the use of glyco-specific antibodies. This approach, termed Nanopoly-BAV, which uses polyamidoamine dendrimers multifunctionalized with boronic acid for specific binding to glycoproteins and with biotin groups for glycoproteins visualization. The Nanopoly-BAV confers femtomolar sensitivity, exceptional glycoprotein specificity and selectivity with as high as 100000 folds for glycoproteins over nonglycoproteins. This synthetic, robust and highly selective Nanopoly-BAV has a great potential to measure cell signaling events by clearly distinguishing actual glycosylation signals from protein expression changes with superior stability. This technique may provide a powerful tool to monitor cellular signaling pathways and discovering new signaling events.

KEYWORDS:

Boronic acid; Dendrimer; Glycoprotein; Visualization on membrane

PMID:
28916104
DOI:
10.1016/j.aca.2017.07.053
[Indexed for MEDLINE]

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