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Mol Immunol. 2017 Nov;91:145-155. doi: 10.1016/j.molimm.2017.09.005. Epub 2017 Sep 12.

Neutralization of viral infectivity by zebrafish c-reactive protein isoforms.

Author information

1
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández (IBMC-UMH). Elche, Spain. Electronic address: melissa.bello@goumh.umh.es.
2
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández (IBMC-UMH). Elche, Spain. Electronic address: alber.falco@umh.es.
3
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández (IBMC-UMH). Elche, Spain. Electronic address: reglita2000@yahoo.com.
4
Investigaciones Marinas.CSIC. Vigo, Spain. Electronic address: patriciapereiro@iim.csic.es.
5
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández (IBMC-UMH). Elche, Spain. Electronic address: jant.encinar@goumh.umh.es.
6
Investigaciones Marinas.CSIC. Vigo, Spain. Electronic address: beatriznovoa@iim.csic.es.
7
Instituto de Biología Molecular y Celular, Universidad Miguel Hernández (IBMC-UMH). Elche, Spain. Electronic address: luis.perez@umh.es.
8
Instituto Nacional Investigaciones y Tecnologías Agrarias y Alimentarias, Dpto. Biotecnología. INIA. Madrid, Spain. Electronic address: juliocoll@inia.es.

Abstract

This work explores the unexpected in vivo and in vitro anti-viral functions of the seven c-reactive protein (crp1-7) genes of zebrafish (Danio rerio). First results showed heterogeneous crp1-7 transcript levels in healthy wild-type zebrafish tissues and organs and how those levels heterogeneously changed not only after bacterial but also after viral infections, including those in adaptive immunity-deficient rag1-/- mutants. As shown by microarray hybridization and proteomic techniques, crp2/CRP2 and crp5/CRP5 transcripts/proteins were among the most modulated during in vivo viral infection situations including the highest responses in the absence of adaptive immunity. In contrast crp1/CRP1/and crp7/CRP7 very often remained unmodulated. All evidences suggested that zebrafish crp2-6/CRP2-6 may have in vivo anti-viral activities in addition to their well known anti-bacterial and/or physiological functions in mammalians. Confirming those expectations, in vitro neutralization and in vivo protection against spring viremia carp virus (SVCV) infections were demonstrated by crp2-6/CRP2-6 using crp1-7 transfected and/or CRP1-7-enriched supernatant-treated fish cells and crp2-5-injected one-cell stage embryo eggs, respectively. All these findings discovered a crp1-7/CRP1-7 primitive anti-viral functional diversity.These findings may help to study similar functions on the one-gene-coded human CRP, which is widely used as a clinical biomarker for bacterial infections, tissue inflammation and coronary heart diseases.

KEYWORDS:

CRP; SVCV; VHSV; anti-viral neutralizing activity; c-reactive protein; microarrays; zebrafish

PMID:
28915434
DOI:
10.1016/j.molimm.2017.09.005
[Indexed for MEDLINE]

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