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Viruses. 2017 Sep 15;9(9). pii: E260. doi: 10.3390/v9090260.

A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice.

Author information

1
Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China. kuixiang@foxmail.com.
2
Department of Pathology, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming 650101, China. kuixiang@foxmail.com.
3
Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany. koussov@vuz.uni-luebeck.de.
4
German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems Site, University of Lübeck, 23562 Lübeck, Germany. koussov@vuz.uni-luebeck.de.
5
China Tobacco Yunnan Industrial Co., Ltd., 41 Keyi Road, Kunming 650106, China. guan_ying1984@163.com.
6
Department of Pathology, The Second Affiliated Hospital of Kunming Medical University, 374 Dianmian Road, Kunming 650101, China. wyan_sky@163.com.
7
Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China. yi_shan@126.com.
8
Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China. wujinyuan@imbcams.com.cn.
9
Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China. yinna@imbcams.com.cn.
10
Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China. yanyan_850@163.com.
11
Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China. lihj6912@hotmail.com.
12
Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Sciences, 935 Jiaoling Road, Kunming 650118, China. maoshs@imbcams.com.cn.

Abstract

Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal-oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459-606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.

KEYWORDS:

combined vaccine; hepatitis A; hepatitis E; neutralization epitope; vector; virus

PMID:
28914805
PMCID:
PMC5618026
DOI:
10.3390/v9090260
[Indexed for MEDLINE]
Free PMC Article

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