Format

Send to

Choose Destination
See comment in PubMed Commons below
J Food Drug Anal. 2017 Jul;25(3):628-636. doi: 10.1016/j.jfda.2016.10.011. Epub 2016 Nov 29.

Long-chain bases from sea cucumber mitigate endoplasmic reticulum stress and inflammation in obesity mice.

Author information

1
Innovation Application Institute, Zhejiang Ocean University, Zhoushan, Zhejiang Province, China; College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China. Electronic address: hushiweihai@163.com.
2
Innovation Application Institute, Zhejiang Ocean University, Zhoushan, Zhejiang Province, China.
3
College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, China.

Abstract

Endoplasmic reticulum (ER) stress and inflammation can induce hyperglycemia. Long-chain bases (LCBs) from sea cucumber exhibit antihyperglycemic activities. However, their effects on ER stress and inflammation are unknown. We investigated the effects of LCBs on ER stress and inflammatory response in high-fat, fructose diet-induced obesity mice. Reactive oxygen species and free fatty acids were measured. Inflammatory cytokines in serum and their mRNA expressions in epididymal adipose tissues were investigated. Hepatic ER stress-related key genes were detected. c-Jun NH2-terminal kinase and nuclear factor κB inflammatory pathways were also evaluated in the liver. Results showed that LCBs reduced serum and hepatic reactive oxygen species and free fatty acids concentrations. LCBs decreased serum proinflammatory cytokines levels, namely interleukin (IL)-1β, tumor necrosis factor-α, IL-6, macrophage inflammatory protein 1, and c-reactive protein, and increased anti-inflammatory cytokine IL-10 concentration. The mRNA and protein expressions of these cytokines in epididymal adipose tissues were regulated by LCBs as similar to their circulatory contents. LCBs inhibited phosphorylated c-Jun NH2-terminal kinase and inhibitor κ kinase β, and nuclear factor κB nuclear translocation. LCBs also inhibited mRNA expression of ER stress markers glucose regulated protein, activating transcription factor 6, double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase, and X-box binding protein 1, and phosphorylation of eukaryotic initiation factor-α and inositol requiring enzyme 1α. These results indicate that LCBs can alleviate ER stress and inflammatory response. Nutritional supplementation with LCBs may offer an adjunctive therapy for RE stress-associated inflammation.

KEYWORDS:

cytokines; endoplasmic reticulum stress; inflammation; long-chain bases; sea cucumber

PMID:
28911649
DOI:
10.1016/j.jfda.2016.10.011
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center