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J Immunol. 2017 Oct 15;199(8):2618-2623. doi: 10.4049/jimmunol.1700888. Epub 2017 Sep 13.

Cutting Edge: Endogenous IFN-β Regulates Survival and Development of Transitional B Cells.

Author information

1
Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294.
2
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294.
3
Toronto General Research Institute, University Health Network, Toronto, Ontario M5G 2C4, Canada.
4
Department of Immunology, University of Toronto, Toronto, Ontario M5G 2M1, Canada; and.
5
Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294; jdmountz@uab.edu.
6
Birmingham Veterans Administration Medical Center, Birmingham, AL 35233.

Abstract

The transitional stage of B cell development is a formative stage in the spleen where autoreactive specificities are censored as B cells gain immune competence, but the intrinsic and extrinsic factors regulating survival of transitional stage 1 (T1) B cells are unknown. We report that B cell expression of IFN-β is required for optimal survival and TLR7 responses of transitional B cells in the spleen and was overexpressed in T1 B cells from BXD2 lupus-prone mice. Single-cell gene expression analysis of B6 Ifnb+/+ versus B6 Ifnb-⁄- T1 B cells revealed heterogeneous expression of Ifnb in wild-type B cells and distinct gene expression patterns associated with endogenous IFN-β. Single-cell analysis of BXD2 T1 B cells revealed that Ifnb is expressed in early T1 B cell development with subsequent upregulation of Tlr7 and Ifna1 Together, these data suggest that T1 B cell expression of IFN-β plays a key role in regulating responsiveness to external factors.

PMID:
28904124
PMCID:
PMC5636672
DOI:
10.4049/jimmunol.1700888
[Indexed for MEDLINE]
Free PMC Article

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