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Small. 2017 Nov;13(41). doi: 10.1002/smll.201700866. Epub 2017 Sep 13.

A DNA Origami Mechanical Device for the Regulation of Microcosmic Structural Rigidity.

Wan N1,2, Hong Z1,2,3, Wang H2,4, Fu X2,4, Zhang Z2,4, Li C2,5, Xia H2,4, Fang Y2,6, Li M4,7, Zhan Y2,3,7,8, Yang X6,7.

Author information

1
National Education Base of Biological Science, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
2
Innovation Base of Life Science and Technology, Qiming College, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
3
Bei Shizhang Advanced Class of Life Science Research, co-founded by Huazhong University of Science and Technology & Institute of Biophysics, Chinese Academy of Sciences & University of Chinese Academy of Sciences, Wuhan, 430074, P. R. China.
4
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
5
Department of Bioinformatics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
6
Department of Nanomedicine and Biopharmaceutics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
7
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.
8
Student Affairs Office, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China.

Abstract

DNA origami makes it feasible to fabricate a tremendous number of DNA nanostructures with various geometries, dimensions, and functionalities. Moreover, an increasing amount of research on DNA nanostructures is focused on biological and biomedical applications. Here, the reversible regulation of microcosmic structural rigidity is accomplished using a DNA origami device in vitro. The designed DNA origami monomer is composed of an internal central axis and an external sliding tube. Due to the external tube sliding, the device transforms between flexible and rigid states. By transporting the device into the liposome, the conformational change of the origami device induces a structural change in the liposome. The results obtained demonstrate that the programmed DNA origami device can be applied to regulate the microcosmic structural rigidity of liposomes. Because microcosmic structural rigidity is important to cell proliferation and function, the results obtained potentially provide a foundation for the regulation of cell microcosmic structural rigidity using DNA nanostructures.

KEYWORDS:

DNA origami; dynamic structure; microcosmic structural rigidity; self-assembly

PMID:
28902974
DOI:
10.1002/smll.201700866

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