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Vaccine. 2017 Oct 9;35(42):5666-5673. doi: 10.1016/j.vaccine.2017.08.044. Epub 2017 Sep 9.

Influenza A haemagglutinin specific IgG responses in children and adults after seasonal trivalent live attenuated influenza vaccination.

Author information

1
Influenza Centre, Department of Clinical Science, University of Bergen, Bergen, Norway; K.G. Jebsen Centre for Influenza Vaccine Research, Department of Clinical Science, University of Bergen, Bergen, Norway.
2
Department of Microbiology, Icahn School of Medicine at Mount Sinai, NY, USA.
3
Influenza Centre, Department of Clinical Science, University of Bergen, Bergen, Norway.
4
Influenza Centre, Department of Clinical Science, University of Bergen, Bergen, Norway; K.G. Jebsen Centre for Influenza Vaccine Research, Department of Clinical Science, University of Bergen, Bergen, Norway; Department of Research & Development, Haukeland University Hospital, Bergen, Norway.
5
Broegelmann Research Laboratory, Department of Clinical Science, University of Bergen, Norway.
6
Influenza Centre, Department of Clinical Science, University of Bergen, Bergen, Norway; K.G. Jebsen Centre for Influenza Vaccine Research, Department of Clinical Science, University of Bergen, Bergen, Norway; Department of Research & Development, Haukeland University Hospital, Bergen, Norway. Electronic address: Rebecca.cox@uib.no.

Abstract

Influenza is a major respiratory pathogen and vaccination is the main method of prophylaxis. In 2012, the trivalent live attenuated influenza vaccine (LAIV3) was licensed in Europe for use in children. Vaccine-induced antibodies directed against the main viral surface glycoprotein, haemagglutinin (HA), play an important role in virus neutralization through different mechanism. The objective of this study was to dissect the HA specific antibody responses induced after LAIV3 immunization to the influenza A viruses in children and adults. Plasma was collected from 20 children and 20 adults pre- and post-LAIV3 vaccination (up to ayear) and analysed by the haemagglutination inhibition (HI) and ELISA assays. We found that LAIV3 boosted the HA specific IgG response against the head and the full-length of H3N2 in children, but not adults. Adults had higher levels of pre-existing stalk antibodies (towards H3N2 and H1N1), but these were not boosted by LAIV3. Importantly, we observed a trend in boosting of H1N1 HA stalk specific antibodies in children after LAIV3. Whereas, heterosubtypic H5 and H7 full-length HA specific antibodies were not boosted in either children or adults. In conclusion, LAIV3 elicited H3-head and low levels of H1 stalk specific antibody responses in children, supporting the prophylactic use of LAIV in children.

KEYWORDS:

Adults; Antibody; Children; HA; HA-stalk; Influenza; LAIV3

PMID:
28899626
DOI:
10.1016/j.vaccine.2017.08.044
[Indexed for MEDLINE]

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