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Bioresour Technol. 2017 Dec;245(Pt A):325-331. doi: 10.1016/j.biortech.2017.08.129. Epub 2017 Aug 31.

Purification and antioxidant activity of phycocyanin from Synechococcus sp. R42DM isolated from industrially polluted site.

Author information

1
Department of Biosciences, UGC-Centre of Advanced Study, Satellite Campus, Vadtal Road, Sardar Patel University, Bakrol 388315, Anand, Gujarat, India.
2
Department of Biotechnology, Shree A. N. Patel PG Institute, Sardar Patel University, Anand 388001, Gujarat, India.
3
Department of Biosciences, UGC-Centre of Advanced Study, Satellite Campus, Vadtal Road, Sardar Patel University, Bakrol 388315, Anand, Gujarat, India. Electronic address: datta_madamwar@yahoo.com.

Abstract

The cyanobacterium Synechococcus sp. R42DM, isolated from an industrially polluted site Vatva, Gujarat, India was recognized to produce phycocyanin (PC) as major phycobiliprotein. In present study, the combinatorial approach of chemical and physical methods i.e. Triton-X 100 treatment and ultra-sonication was designed for extraction of PC. From cell extract, the intact and functional-PC was purified up to purity 4.03 by ammonium sulphate fractionation and ion-exchange chromatography. The PC displayed considerable in vitro antioxidant and radical-scavenging activity. This PC was further noticed to scavenge intracellular-ROS and to increase tolerance against thermal and oxidative stress in Caenorhabditis elegans. Moreover, the PC was noticed to improve the physiological behaviour and longevity of C. elegans. In addition, the PC showed remarkable stability under physico-chemical stressors, which is desirable for their use in biomedical applications. In conclusion, present paper added up evidence in support of the prospective use of PC as an antioxidant nutraceutical.

KEYWORDS:

Antioxidant; Caenorhabditis elegans; Cyanobacteria; Phycocyanin; Reactive oxygen species

PMID:
28898827
DOI:
10.1016/j.biortech.2017.08.129
[Indexed for MEDLINE]

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