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Macromol Biosci. 2017 Nov;17(11). doi: 10.1002/mabi.201700185. Epub 2017 Sep 12.

Design of a Novel Two-Component Hybrid Dermal Scaffold for the Treatment of Pressure Sores.

Author information

1
Regenerative Biomaterials Group, RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood, HA6 2RN, UK.
2
Department of Biochemical Engineering, University College London, Gower Street, London, WC1E 6BT, UK.
3
Institute of Child Health, University College London, UCL Great Ormond Street, 30 Guilford Street, London, WC1N 1EH, UK.

Abstract

The aim of this study is to design a novel two-component hybrid scaffold using the fibrin/alginate porous hydrogel Smart Matrix combined to a backing layer of plasma polymerized polydimethylsiloxane (Sil) membrane to make the fibrin-based dermal scaffold more robust for the treatment of the clinically challenging pressure sores. A design criteria are established, according to which the Sil membranes are punched to avoid collection of fluid underneath. Manual peel test shows that native silicone does not attach to the fibrin/alginate component while the plasma polymerized silicone membranes are firmly bound to fibrin/alginate. Structural characterization shows that the fibrin/alginate matrix is intact after the addition of the Sil membrane. By adding a Sil membrane to the original fibrin/alginate scaffold, the resulting two-component scaffolds have a significantly higher shear or storage modulus G'. In vitro cell studies show that dermal fibroblasts remain viable, proliferate, and infiltrate the two-component hybrid scaffolds during the culture period. These results show that the design of a novel two-component hybrid dermal scaffold is successful according to the proposed design criteria. To the best of the authors' knowledge, this is the first study that reports the combination of a fibrin-based scaffold with a plasma-polymerized silicone membrane.

KEYWORDS:

dermal scaffold; fibrin; hybrid; pressure sores; silicone

PMID:
28895290
DOI:
10.1002/mabi.201700185
[Indexed for MEDLINE]

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