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Development. 2017 Oct 15;144(20):3798-3807. doi: 10.1242/dev.150516. Epub 2017 Sep 11.

Timing of adrenal regression controlled by synergistic interaction between Sf1 SUMOylation and Dax1.

Author information

1
Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan Health System, Ann Arbor, MI 48109-2200, USA.
2
Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
3
Department of Cellular Molecular Pharmacology, School of Medicine, University of California, San Francisco, CA 94158, USA.
4
Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan Health System, Ann Arbor, MI 48109-2200, USA ghammer@umich.edu.

Abstract

The nuclear receptor steroidogenic factor 1 (Sf1, Nr5a1, Ad4bp) is crucial for formation, development and function of steroidogenic tissues. A fetal adrenal enhancer (FAdE) in the Sf1 gene was previously identified to direct Sf1 expression exclusively in the fetal adrenal cortex and is bound by both Sf1 and Dax1. Here, we have examined the function of Sf1 SUMOylation and its interaction with Dax1 on FAdE function. A diffused prolonged pattern of FAdE expression and delayed regression of the postnatal fetal cortex (X-zone) were detected in both the SUMOylation-deficient-Sf12KR/2KR and Dax1 knockout mouse lines, with FAdE expression/activity retained in the postnatal 20αHSD-positive postnatal X-zone cells. In vitro studies indicated that Sf1 SUMOylation, although not directly influencing DNA binding, actually increased binding of Dax1 to Sf1 to further enhance transcriptional repression of FAdE. Taken together, these studies define a crucial repressor function of Sf1 SUMOylation and Dax1 in the physiological cessation of FAdE-mediated Sf1 expression and the resultant regression of the postnatal fetal cortex (X-zone).

KEYWORDS:

Adrenal gland; Nuclear receptors; SUMOylation; X-zone

PMID:
28893949
PMCID:
PMC5675444
DOI:
10.1242/dev.150516
[Indexed for MEDLINE]
Free PMC Article

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