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Bioresour Technol. 2018 Jan;247:1184-1188. doi: 10.1016/j.biortech.2017.08.166. Epub 2017 Sep 1.

Improved production of 2,5-furandicarboxylic acid by overexpression of 5-hydroxymethylfurfural oxidase and 5-hydroxymethylfurfural/furfural oxidoreductase in Raoultella ornithinolytica BF60.

Author information

1
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
2
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA.
3
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
4
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address: zpshi@jiangnan.edu.cn.

Abstract

2,5-Furandicarboxylic acid (FDCA) is a promising bio-based building block and can be produced by biotransformation of 5-hydroxymethylfurfural (HMF). To improve the FDCA production, two genes-one encoding HMF oxidase (HMFO; from Methylovorus sp. strain MP688) and another encoding for HMF/Furfural oxidoreductase (HmfH; from Cupriavidus basilensis HMF14)-were introduced into Raoultella ornithinolytica BF60. The FDCA production in the engineered whole-cell biocatalyst increased from 51.0 to 93.6mM, and the molar conversion ratio of HMF to FDCA increased from 51.0 to 93.6%.

KEYWORDS:

2,5-Furandicarboxylic acid; 5-Hydroxymethylfurfural oxidase; Biotransformation; HMF/furfural oxidoreductase; Raoultella ornithinolytica BF60

PMID:
28893500
DOI:
10.1016/j.biortech.2017.08.166
[Indexed for MEDLINE]

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