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ACS Synth Biol. 2018 Jan 19;7(1):209-217. doi: 10.1021/acssynbio.7b00279. Epub 2017 Oct 3.

Cloning and Transplantation of the Mesoplasma florum Genome.

Author information

1
Université de Sherbrooke , Département de Biologie, 2500 Boulevard Université, Sherbrooke, Québec J1K 2R1, Canada.
2
Université de Bordeaux , INRA, UMR 1332 de Biologie du Fruit et Pathologie, F-33140 Villenave d'Ornon, France.

Abstract

Cloning and transplantation of bacterial genomes is a powerful method for the creation of engineered microorganisms. However, much remains to be understood about the molecular mechanisms and limitations of this approach. We report the whole-genome cloning of Mesoplasma florum in Saccharomyces cerevisiae, and use this model to investigate the impact of a bacterial chromosome in yeast cells. Our results indicate that the cloned M. florum genome is subjected to weak transcriptional activity, and causes no significant impact on yeast growth. We also report that the M. florum genome can be transplanted into Mycoplasma capricolum without any negative impact from the putative restriction enzyme encoding gene mfl307. Using whole-genome sequencing, we observed that a small number of mutations appeared in all M. florum transplants. Mutations also arose, albeit at a lower frequency, when the M. capricolum genome was transplanted into M. capricolum recipient cells. These observations suggest that genome transplantation is mutagenic, and that this phenomenon is magnified by the use of genome donor and recipient cell belonging to different species. No difference in efficiency was detected after three successive rounds of genome transplantation, suggesting that the observed mutations were not selected during the procedure. Taken together, our results provide a more accurate picture of the events taking place during bacterial genome cloning and transplantation.

KEYWORDS:

Mesoplasma florum; Saccharomyces cerevisiae; genome transplantation; whole-genome cloning

PMID:
28893065
DOI:
10.1021/acssynbio.7b00279
[Indexed for MEDLINE]

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