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Int J Mol Sci. 2017 Sep 11;18(9). pii: E1948. doi: 10.3390/ijms18091948.

Antioxidant Artemisia princeps Extract Enhances the Expression of Filaggrin and Loricrin via the AHR/OVOL1 Pathway.

Author information

1
Beauty Care R&D, Health & Beauty Company, Sunstar Group, Kamihamuro 5-30-1, Takatsuki, Osaka 569-1044, Japan. akiko.hirano@jp.sunstar.com.
2
Beauty Care R&D, Health & Beauty Company, Sunstar Group, Kamihamuro 5-30-1, Takatsuki, Osaka 569-1044, Japan. masashi.goto@jp.sunstar.com.
3
Beauty Care R&D, Health & Beauty Company, Sunstar Group, Kamihamuro 5-30-1, Takatsuki, Osaka 569-1044, Japan. tsukasa.mitsui@jp.sunstar.com.
4
Department of Dermatology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. ahachi@dermatol.med.kyushu-u.ac.jp.
5
Department of Dermatology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. gakku@dermatol.med.kyushu-u.ac.jp.
6
Department of Dermatology, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. furue@dermatol.med.kyushu-u.ac.jp.

Abstract

The Japanese mugwort, Artemisia princeps (yomogi in Japanese), has anti-inflammatory and antioxidant effects. Skin care products containing Artemisia princeps extract (APE) are known to improve dry skin symptoms in atopic dermatitis. Atopic dry skin is associated with a marked reduction of skin barrier proteins, such as filaggrin (FLG) and loricrin (LOR). Recently, aryl hydrocarbon receptor (AHR), and its downstream transcription factor OVO-like 1 (OVOL1), have been shown to regulate the gene expression of FLG and LOR. The focus of this paper is to evaluate the effects of APE on the AHR/OVOL1/FLG or LOR pathway since they have remained unknown to this point. We first demonstrated that non-cytotoxic concentrations of APE significantly upregulated antioxidant enzymes, NAD(P)H dehydrogenase quinone 1 and heme oxygenase 1, in human keratinocytes. Even at these low concentrations, APE induced nuclear translocation of AHR and significantly upregulated CYP1A1 (a specific target gene for AHR activation), FLG, and LOR expression. AHR knockdown downregulated OVOL1 expression. The APE-induced upregulation of FLG and LOR was canceled in keratinocytes with AHR or OVOL1 knockdown. In conclusion, antioxidant APE is a potent phytoextract that upregulates FLG and LOR expression in an AHR/OVOL1-dependent manner and this may underpin the barrier-repairing effects of APE in treating atopic dry skin.

KEYWORDS:

Artemisia princeps extract; OVO-like 1; aryl hydrocarbon receptor; filaggrin; loricrin; nuclear factor-erythroid 2-related factor-2

PMID:
28892018
PMCID:
PMC5618597
DOI:
10.3390/ijms18091948
[Indexed for MEDLINE]
Free PMC Article

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