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Phytomedicine. 2017 Sep 15;33:7-13. doi: 10.1016/j.phymed.2017.05.009. Epub 2017 Jul 8.

Anti-adipogenesis mechanism of pterostilbene through the activation of heme oxygenase-1 in 3T3-L1 cells.

Author information

1
Department of Food Science and Biotechnology, College of Life Science, CHA University, CHA Biocomplex, 335 Pangyo-ro, Bundang-gu, Seongnam, Kyonggi 463-400, Republic of Korea.
2
Department of Food Science and Biotechnology, College of Life Science, CHA University, CHA Biocomplex, 335 Pangyo-ro, Bundang-gu, Seongnam, Kyonggi 463-400, Republic of Korea. Electronic address: bylee@cha.ac.kr.

Abstract

BACKGROUND:

Pterostilbene is a stilbenoid and major compound and has diverse biological activities, such as antioxidant, anti-cancer, and anti-inflammatory. However, it has not been shown whether pterostilbene affects the mitotic clonal expansion during adipogenesis in 3T3-L1 cells.

PURPOSE:

In the present study, we aimed to demonstrate the detailed mechanism of pterostilbene on anti-adipogenesis in 3T3-L1 cells.

METHODS:

Preadipocytes were converted to adipocytes through treatment with MDI (IBMX; 3-isobutyl-1-methylxanthine, DEX; dexamethasone, insulin) in 3T3-L1 cells. Oil Red O staining was performed to measure intracellular lipid accumulation. Western blot analysis was conducted to analyze protein expressions.

RESULTS:

Our results showed that pterostilbene decreased the lipid accumulation compared to MDI-induced differentiation, using Oil Red O staining. Next, we found that pterostilbene suppressed the expression of C/EBPα, PPARγ, and aP2 as well as the mitotic clonal expansion-associated proteins CHOP10 and C/EBPβ, by western blot analysis. Our results indicated that pterostilbene may repress adipocyte differentiation through the activation of HO-1 expression prior to entering into the mitotic clonal expansion in 3T3-L1 cells. RNA interference was used to determine whether HO-1 acts as a regulator of CHOP10.

CONCLUSION:

Our results revealed that pterostilbene induced HO-1 expression which acts as a regulator of CHOP10. Together, we demonstrated that pterostilbene suppresses the initiation of mitotic clonal expansion via up-regulation of HO-1 expression during adipocyte differentiation of 3T3-L1 cells.

KEYWORDS:

Adipocytes; Adipogenesis; CHOP10; Heme oxygenase-1; Pterostilbene

PMID:
28887923
DOI:
10.1016/j.phymed.2017.05.009
[Indexed for MEDLINE]

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