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Sci Rep. 2017 Sep 8;7(1):10966. doi: 10.1038/s41598-017-11298-7.

Identification of immunity-related genes in Plutella xylostella in response to fungal peptide destruxin A: RNA-Seq and DGE analysis.

Author information

1
College of Agriculture, South China Agricultural University, Laboratory of Bio-Pesticide Creation and Application of Guangdong Province, Guangzhou, P.R. China.
2
State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
3
BGI-Shenzhen, Shenzhen, P.R. China.
4
School of Biological Sciences, University of Missouri-Kansas, Kansas City, MO, 64110, USA.
5
College of Agriculture, South China Agricultural University, Laboratory of Bio-Pesticide Creation and Application of Guangdong Province, Guangzhou, P.R. China. jflbang@scau.edu.cn.

Abstract

Plutella xylostella has become the major lepidopteran pest of Brassica owing to its strong ability of resistance development to a wide range of insecticides. Destruxin A, a mycotoxin of entomopathogenic fungus, Metarhizium anisopliae, has broad-spectrum insecticidal effects. However, the interaction mechanism of destruxin A with the immune system of P. xylostella at genomic level is still not well understood. Here, we identified 129 immunity-related genes, including pattern recognition receptors, signal modulators, few members of main immune pathways (Toll, Imd, and JAK/STAT), and immune effectors in P. xylostella in response to destruxin A at three different time courses (2 h, 4 h, and 6 h). It is worthy to mention that the immunity-related differentially expressed genes (DEGs) analysis exhibited 30, 78, and 72 up-regulated and 17, 13, and 6 down-regulated genes in P. xylostella after destruxin A injection at 2 h, 4 h, and 6 h, respectively, compared to control. Interestingly, our results revealed that the expression of antimicrobial peptides that play a vital role in insect immune system was up-regulated after the injection of destruxin A. Our findings provide a detailed information on immunity-related DEGs and reveal the potential of P. xylostella to limit the infection of fungal peptide destruxin A by increasing the activity of antimicrobial peptides.

PMID:
28887550
PMCID:
PMC5591186
DOI:
10.1038/s41598-017-11298-7
[Indexed for MEDLINE]
Free PMC Article

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