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Sci Rep. 2017 Sep 8;7(1):11054. doi: 10.1038/s41598-017-11351-5.

MiRNA-target network analysis identifies potential biomarkers for Traditional Chinese Medicine (TCM) syndrome development evaluation in hepatitis B caused liver cirrhosis.

Author information

1
Research Center for TCM Complexity System, Shanghai University of TCM, Shanghai, 201203, China.
2
School of Clinical Medicine, Shanghai University of Medicine & Health Sciences, Shanghai, 201203, China.
3
Shu Guang Hospital Affiliated to Shanghai University of TCM, Shanghai, 201203, China.
4
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
5
Research Center for TCM Complexity System, Shanghai University of TCM, Shanghai, 201203, China. shibingsu07@163.com.
6
Research Center for TCM Complexity System, Shanghai University of TCM, Shanghai, 201203, China. cqlw1975@126.com.

Abstract

Hepatitis B is one of most etiologies of Liver cirrhosis in China, and clinically lacks the effective strategy for Hepatitis B caused cirrhosis (HBC) therapy. As a complementary and alternative medicine, Chinese Traditional Medicine (TCM) has special therapeutic effects for HBC. Here, we focus on the evolution process of HBC TCM syndromes, which was from Excessive (Liver-Gallbladder Dampness-Heat Syndrome, LGDHS) to Deficient (Liver-Kidney Deficiency Syndrome, LKYDS) via Excessive-Deficient syndrome (Liver-Depression and Spleen-Deficiency Syndrome, LDSDS). Using R package, 16 miRNAs in LGDHS/Normal, 48 miRNAs in LDSDS/LGDHS, and 16 miRNAs in LKYDS/LDSDS were identified, respectively. The miRNA-target networks show that the LDSDS was most stability and complicated. Subsequently, 4 kernel miRNAs with LGDHS-LDSDS process, and 5 kernel miRNAs with LDSDS-LKYDS process were screened. Using RT-qPCR data, p1 (hsa-miR-17-3p, -377-3p, -410-3p and -495) and p2 miRNA panel (hsa-miR-377-3p, -410-3p, -27a-3p, 149-5p and 940) were identified by Logistic Regression Model, which clearly improve the accuracy of TCM syndrome classification. The rebuilt miRNA-target network shows that the LDSDS is a critical point and might determine the evolution directions of HBC TCM syndrome. This study suggests that the identified kernel miRNAs act as potential biomarkers and benefit to evaluate the evolution tendency of HBC TCM syndromes.

PMID:
28887510
PMCID:
PMC5591282
DOI:
10.1038/s41598-017-11351-5
[Indexed for MEDLINE]
Free PMC Article

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