Format

Send to

Choose Destination
Mol Phylogenet Evol. 2017 Nov;116:213-226. doi: 10.1016/j.ympev.2017.08.020. Epub 2017 Sep 6.

Transcriptome and target DNA enrichment sequence data provide new insights into the phylogeny of vespid wasps (Hymenoptera: Aculeata: Vespidae).

Author information

1
Center for Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, Adenauerallee 160, 53113 Bonn, Germany.
2
Center for Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, Adenauerallee 160, 53113 Bonn, Germany; Department of Evolutionary Biology and Ecology, Institute for Biology I (Zoology), University of Freiburg, Hauptstraße 1, 79104 Freiburg, Germany.
3
Institute of Evolutionary Biology and Ecology, University of Bonn, An der Immenburg 1, 53121 Bonn, Germany.
4
Scientific Computing Group, Heidelberg Institute for Theoretical Studies, Schloss-Wolfsbrunnenweg 35, 69118 Heidelberg, Germany.
5
Department of Entomology, State Museum of Natural History, Rosenstein 1, 70191 Stuttgart, Germany.
6
National University of Singapore, 14 Science Dr 4, Singapore 117543, Singapore.
7
Via Belvedere 8/d, 20044 Bernareggio MI, Italy.
8
Department of Animal Ecology and Tropical Biology, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
9
Department of Evolutionary Biology and Ecology, Institute for Biology I (Zoology), University of Freiburg, Hauptstraße 1, 79104 Freiburg, Germany; Department of Animal Ecology and Tropical Biology, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
10
China National GeneBank-Shenzhen, BGI-Shenzhen, Shenzhen, Guangdong Province 518083, People's Republic of China; BGI-Shenzhen, Shenzhen, Guangdong Province 518083, People's Republic of China; Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350 Copenhagen, Denmark.
11
Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing 100193, People's Republic of China; Department of Entomology, China Agricultural University, Beijing 100193, People's Republic of China.
12
Center of Taxonomy and Evolutionary Research, Arthropoda Department, Zoological Research Museum Alexander Koenig, Adenauerallee 160, 53113 Bonn, Germany. Electronic address: r.peters@leibniz-zfmk.de.
13
Center for Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, Adenauerallee 160, 53113 Bonn, Germany; Department of Evolutionary Biology and Ecology, Institute for Biology I (Zoology), University of Freiburg, Hauptstraße 1, 79104 Freiburg, Germany. Electronic address: oliver.niehuis@biologie.uni-freiburg.de.

Abstract

The wasp family Vespidae comprises more than 5000 described species which represent life history strategies ranging from solitary and presocial to eusocial and socially parasitic. The phylogenetic relationships of the major vespid wasp lineages (i.e., subfamilies and tribes) have been investigated repeatedly by analyzing behavioral and morphological traits as well as nucleotide sequences of few selected genes with largely incongruent results. Here we reconstruct their phylogenetic relationships using a phylogenomic approach. We sequenced the transcriptomes of 24 vespid wasp and eight outgroup species and exploited the transcript sequences for design of probes for enriching 913 single-copy protein-coding genes to complement the transcriptome data with nucleotide sequence data from additional 25 ethanol-preserved vespid species. Results from phylogenetic analyses of the combined sequence data revealed the eusocial subfamily Stenogastrinae to be the sister group of all remaining Vespidae, while the subfamily Eumeninae turned out to be paraphyletic. Of the three currently recognized eumenine tribes, Odynerini is paraphyletic with respect to Eumenini, and Zethini is paraphyletic with respect to Polistinae and Vespinae. Our results are in conflict with the current tribal subdivision of Eumeninae and thus, we suggest granting subfamily rank to the two major clades of "Zethini": Raphiglossinae and Zethinae. Overall, our findings corroborate the hypothesis of two independent origins of eusociality in vespid wasps and suggest a single origin of using masticated and salivated plant material for building nests by Raphiglossinae, Zethinae, Polistinae, and Vespinae. The inferred phylogenetic relationships and the open access vespid wasp target DNA enrichment probes will provide a valuable tool for future comparative studies on species of the family Vespidae, including their genomes, life styles, evolution of sociality, and co-evolution with other organisms.

KEYWORDS:

Eumeninae; Phylogenomics; RNA-seq; Raphiglossinae; Transcriptomics; Zethinae

PMID:
28887149
DOI:
10.1016/j.ympev.2017.08.020
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center