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World J Gastroenterol. 2017 Aug 21;23(31):5669-5679. doi: 10.3748/wjg.v23.i31.5669.

Fluctuation of zonulin levels in blood vs stability of antibodies.

Author information

1
Department of Preventive Medicie, Loma Linda University, Evans Hall, Loma Linda, CA 92354, United States.
2
Regenera Wellness, Los Angeles, CA 90025 United States.
3
Harvard Medical School, Boston, MA 02115, United States.

Abstract

AIM:

To evaluate the measurement of zonulin level and antibodies of zonulin and other tight junction proteins in the blood of controls and celiac disease patients.

METHODS:

This study was conducted to assess the variability or stability of zonulin levels vs IgA and IgG antibodies against zonulin in blood samples from 18 controls at 0, 6, 24 and 30 h after blood draw. We also measured zonulin level as well as zonulin, occludin, vinculin, aquaporin 4 and glial fibrillary acidic protein antibodies in the sera of 30 patients with celiac disease and 30 controls using enzyme-linked immunosorbent assay methodology.

RESULTS:

The serum zonulin level in 6 out of 18 subjects was low or < 2.8 ng/mL and was very close to the detection limit of the assay. The other 12 subjects had zonulin levels of > 2.8 ng/mL and showed significant fluctuation from sample to sample. Comparatively, zonulin antibody measured in all samples was highly stable and reproducible from sample to sample. Celiac disease patients showed zonulin levels with a mean of 8.5 ng/mL compared to 3.7 ng/mL in controls (P < 0.0001). Elevation of zonulin level at 2SD above the mean was demonstrated in 37% of celiac disease patients, while antibodies against zonulin, occludin and other tight junction proteins was detected in up to 86% of patients with celiac disease.

CONCLUSION:

Due to its fluctuation, a single measurement of zonulin level is not recommended for assessment of intestinal barrier integrity. Measurement of IgG and IgA antibodies against zonulin, occludin, and other tight junction proteins is proposed for the evaluation of the loss of intestinal barrier integrity.

KEYWORDS:

Celiac disease; Intestinal barrier integrity; Occludin; Tight junction protein; Zonulin

PMID:
28883692
PMCID:
PMC5569281
DOI:
10.3748/wjg.v23.i31.5669
[Indexed for MEDLINE]
Free PMC Article

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