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Science. 2017 Nov 3;358(6363):672-676. doi: 10.1126/science.aan6516. Epub 2017 Sep 7.

The condensin complex is a mechanochemical motor that translocates along DNA.

Author information

1
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
2
Cell Biology and Biophysics Unit, Structural and Computational Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
3
Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, Netherlands.
4
Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, Netherlands. c.dekker@tudelft.nl christian.haering@embl.de ecg2108@cumc.columbia.edu.
5
Cell Biology and Biophysics Unit, Structural and Computational Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. c.dekker@tudelft.nl christian.haering@embl.de ecg2108@cumc.columbia.edu.
6
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA. c.dekker@tudelft.nl christian.haering@embl.de ecg2108@cumc.columbia.edu.

Abstract

Condensin plays crucial roles in chromosome organization and compaction, but the mechanistic basis for its functions remains obscure. We used single-molecule imaging to demonstrate that Saccharomyces cerevisiae condensin is a molecular motor capable of adenosine triphosphate hydrolysis-dependent translocation along double-stranded DNA. Condensin's translocation activity is rapid and highly processive, with individual complexes traveling an average distance of ≥10 kilobases at a velocity of ~60 base pairs per second. Our results suggest that condensin may take steps comparable in length to its ~50-nanometer coiled-coil subunits, indicative of a translocation mechanism that is distinct from any reported for a DNA motor protein. The finding that condensin is a mechanochemical motor has important implications for understanding the mechanisms of chromosome organization and condensation.

Comment in

PMID:
28882993
PMCID:
PMC5862036
DOI:
10.1126/science.aan6516
[Indexed for MEDLINE]
Free PMC Article

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