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In Vivo. 2017 Sep-Oct;31(5):895-903.

Osteochondral Repair Using Porous Three-dimensional Nanocomposite Scaffolds in a Rabbit Model.

Author information

1
Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland beatazyl@wp.pl.
2
Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Cracow, Poland.
3
Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
4
Department of Histology and Embryology, Laboratory for Experimental Cytology, Medical University of Lublin, Lublin, Poland.
5
Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences in Lublin, Lublin, Poland.
6
Department of Biology and Environmental Hygiene, University of Life Sciences in Lublin, Lublin, Poland.

Abstract

AIM:

To evaluate the utility of a novel nanocomposite biomaterial consisting of poly-L/D-lactide, and hydroxyapatite bioceramics, enriched with sodium alginate in articular cartilage defect treatment.

MATERIALS AND METHODS:

The biomaterial was prepared using the method of solvent casting and particle leaching. The study was conducted on 20 New Zealand White rabbits. Experimental osteochondral defects were created in the femoral trochlear grooves and filled with biomaterials. In control groups, the defects were left to spontaneously heal. The quality of newly-formed tissue was evaluated on the basis of macroscopic and histological assessment. Additionally the level of osteogenic and cartilage degradation markers were measured.

RESULTS:

The majority of the defects from the treatment group were covered with tissue similar in structure and colour to healthy cartilage, whereas in the control group, tissue was uneven, and not integrated into the surrounding cartilage.

CONCLUSION:

The results obtained validate the choice of biomaterial used in this study as well as the method of its application.

KEYWORDS:

Cartilage; biomaterial; femoral trochlea; osteochondral defect; poly-L/D-lactide; rabbit; tissue regeneration

PMID:
28882956
PMCID:
PMC5656863
[Indexed for MEDLINE]
Free PMC Article

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