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In Vivo. 2017 Sep-Oct;31(5):779-791.

Tissue-directed Implantation Using Ultrasound Visualization for Development of Biologically Relevant Metastatic Tumor Xenografts.

Author information

1
Department of Surgery, C.S Mott Children's and Women's Hospital, Mott Solid Tumor Oncology Program, The University of Michigan Medical School, Ann Arbor, MI, U.S.A.
2
Department of Pathology, C.S Mott Children's and Women's Hospital, Mott Solid Tumor Oncology Program, The University of Michigan Medical School, Ann Arbor, MI, U.S.A.
3
Unit for Laboratory Animal Medicine, The University of Michigan Medical School, Ann Arbor, MI, U.S.A.
4
Department of Radiology, C.S Mott Children's and Women's Hospital, Mott Solid Tumor Oncology Program, The University of Michigan Medical School, Ann Arbor, MI, U.S.A.
5
Department of Pediatrics, C.S Mott Children's and Women's Hospital, Mott Solid Tumor Oncology Program, The University of Michigan Medical School, Ann Arbor, MI, U.S.A.
6
Department of Surgery, C.S Mott Children's and Women's Hospital, Mott Solid Tumor Oncology Program, The University of Michigan Medical School, Ann Arbor, MI, U.S.A. eanewman@med.umich.edu.

Abstract

BACKGROUND:

Advances in cancer therapeutics depend on reliable in vivo model systems. To develop biologically relevant xenografts, ultrasound was utilized for tissue-directed implantation of neuroblastoma (NB) cell line and patient-derived tumors in the adrenal gland, and for renal subcapsular engraftment of Ewing's sarcoma (ES).

MATERIALS AND METHODS:

NB xenografts were established by direct adrenal injection of luciferase-transfected NB cell lines (IMR32, SH-SY5Y, SK-N-BE2) or NB patient-derived tumor cells (UMNBL001, UMNBL002). ES xenografts were established by renal subcapsular injection of TC32, A673, CHLA-25, or A4573 cells. Progression was monitored by in vivo imaging.

RESULTS:

Tumors progressed to local disease with metastasis evident by 5 weeks. Metastatic sites included cortical bone, lung, liver, and lymph nodes. Xenografted tumors retained immunochemical features of the original cancer.

CONCLUSION:

Human NB adrenal xenografts, including two patient-derived orthotopic, and ES renal subcapsular xenografts were established by ultrasound without open surgery. Tissue-directed implantation is an effective technique for developing metastatic preclinical models.

KEYWORDS:

Ewing's sarcoma; neuroblastoma; orthotopic; preclinical model; ultrasound; xenograft

PMID:
28882943
PMCID:
PMC5656850
[Indexed for MEDLINE]
Free PMC Article

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