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Diabetes Obes Metab. 2017 Sep;19 Suppl 1:107-114. doi: 10.1111/dom.13015.

A 4D view on insulin secretory granule turnover in the β-cell.

Müller A1,2,3, Mziaut H1,2,3, Neukam M1,2,3, Knoch KP1,2,3, Solimena M1,2,3,4.

Author information

1
Molecular Diabetology, University Hospital and Faculty of Medicine Carl Gustav Carus, TU Dresden, Dresden, Germany.
2
Paul Langerhans Institute Dresden (PLID) of the Helmholtz Center Munich, University Hospital Carl Gustav Carus and Faculty of Medicine of the TU Dresden, Dresden, Germany.
3
German Center for Diabetes Research (DZD e.V.), Neuherberg, Germany.
4
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany.

Abstract

Insulin secretory granule (SG) turnover consists of several highly regulated processes allowing for proper β-cell function and insulin secretion. Besides the spatial distribution of insulin SGs, their age has great impact on the likelihood of their secretion and their behaviour within the β-cell. While quantitative measurements performed decades ago demonstrated the preferential secretion of young insulin, new experimental approaches aim to investigate insulin ageing at the granular level. Live-cell imaging, automated image analysis and correlative light and electron microscopy have fostered knowledge of age-defined insulin SG dynamics, their interaction with the cytoskeleton and ultrastructural features. Here, we review our recent work in regards to the connection between insulin SG age, SG dynamics, intracellular location and interaction with other proteins.

KEYWORDS:

SNAP tag; insulin degradation; insulin secretion; insulin secretory granule ageing; microscopy

PMID:
28880479
DOI:
10.1111/dom.13015
[Indexed for MEDLINE]

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