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J Dent Anesth Pain Med. 2016 Dec;16(4):295-302. doi: 10.17245/jdapm.2016.16.4.295. Epub 2016 Dec 31.

Dexmedetomidine attenuates H2O2-induced cell death in human osteoblasts.

Author information

1
Department of Dental Anesthesia and Pain Medicine, School of Dentistry, Pusan National University, Dental Research Institute, Yangsan, Republic of Korea.
2
Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan, Republic of Korea.
3
Department of Anesthesia and Pain Medicine, School of Medicine, Pusan National University, Yangsan, Republic of Korea.

Abstract

BACKGROUND:

Reactive oxygen species play critical roles in homeostasis and cell signaling. Dexmedetomidine, a specific agonist of the α2-adrenoceptor, has been commonly used for sedation, and it has been reported to have a protective effect against oxidative stress. In this study, we investigated whether dexmedetomidine has a protective effect against H2O2-induced oxidative stress and the mechanism of H2O2-induced cell death in normal human fetal osteoblast (hFOB) cells.

METHODS:

Cells were divided into three groups: control group-cells were incubated in normoxia without dexmedetomidine, hydrogen peroxide (H2O2) group-cells were exposed to H2O2 (200 µM) for 2 h, and Dex/H2O2 group-cells were pretreated with dexmedetomidine (5 µM) for 2 h then exposed to H2O2 (200 µM) for 2 h. Cell viability and apoptosis were evaluated. Osteoblast maturation was determined by assaying bone nodular mineralization. Expression levels of bone-related proteins were determined by western blot.

RESULTS:

Cell viability was significantly decreased in the H2O2 group compared with the control group, and this effect was improved by dexmedetomidine. The Hoechst 33342 and Annexin-V FITC/PI staining revealed that dexmedetomidine effectively decreased H2O2-induced hFOB cell apoptosis. Dexmedetomidine enhanced the mineralization of hFOB cells when compared to the H2O2 group. In western blot analysis, bone-related protein was increased in the Dex/H2O2 group.

CONCLUSIONS:

We demonstrated the potential therapeutic value of dexmedetomidine in H2O2-induced oxidative stress by inhibiting apoptosis and enhancing osteoblast activity. Additionally, the current investigation could be evidence to support the antioxidant potential of dexmedetomidine in vitro.

KEYWORDS:

Dexmedetomidine; Osteoblasts; Oxidative stress

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