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J Immunol. 2017 Oct 15;199(8):2631-2638. doi: 10.4049/jimmunol.1700615. Epub 2017 Sep 6.

Activation of Human Mucosal-Associated Invariant T Cells Induces CD40L-Dependent Maturation of Monocyte-Derived and Primary Dendritic Cells.

Author information

1
Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom; mariolina.salio@imm.ox.ac.uk.
2
Malaghan Institute of Medical Research, School of Biological Sciences, Victoria University of Wellington, Wellington 6242, New Zealand.
3
Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom.
4
School of Biosciences, University of Birmingham, Birmingham B11 2TT, United Kingdom.
5
The Ferrier Research Institute, Victoria University of Wellington, Lower Hutt 5046, New Zealand; and.
6
Maurice Wilkins Centre for Molecular Biodiscovery, Auckland 1042, New Zealand.

Abstract

Mucosal-associated invariant T (MAIT) cells are innate T cells that recognize intermediates of the vitamin B2 biosynthetic pathway presented by the monomorphic MR1 molecule. It remains unclear whether, in addition to their cytolytic activity that is important in antimicrobial defense, MAIT cells have immune-modulatory functions that could enhance dendritic cell (DC) maturation. In this study, we investigated the molecular mechanisms dictating the interactions between human MAIT cells and DCs and demonstrate that human MAIT cells mature monocyte-derived and primary DCs in an MR1- and CD40L-dependent manner. Furthermore, we show that MAIT cell-derived signals synergize with microbial stimuli to induce secretion of bioactive IL-12 by DCs. Activation of human MAIT cells in whole blood leads to MR1- and cytokine-dependent NK cell transactivation. Our results underscore an important property of MAIT cells, which can be of translational relevance to rapidly orchestrate adaptive immunity through DC maturation.

PMID:
28877992
PMCID:
PMC5632842
DOI:
10.4049/jimmunol.1700615
[Indexed for MEDLINE]
Free PMC Article

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