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J Biomed Mater Res A. 2017 Dec;105(12):3422-3431. doi: 10.1002/jbm.a.36193. Epub 2017 Sep 26.

Polyester vascular patches acquire arterial or venous identity depending on their environment.

Bai H1,2,3,4, Hu H2,3, Guo J2,3, Ige M2,3, Wang T2,3, Isaji T2,3, Kudze T2,3, Liu H2,3, Yatsula B2,3, Hashimoto T2,3, Xing Y1, Dardik A2,3,5.

Author information

1
Department of Physiology, Basic Medical College of Zhengzhou University, Henan, China.
2
The Vascular Biology and Therapeutics Program, Yale University School of Medicine, New Haven, Connecticut.
3
Department of Surgery, Yale University School of Medicine, New Haven, Connecticut.
4
Department of Vascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China.
5
Department of Surgery, VA Connecticut Healthcare System, West Haven, Connecticut.

Abstract

Polyester is commonly used in vascular surgery for patch angioplasty and grafts. We hypothesized that polyester patches heal by infiltration of arterial or venous progenitor cells depending on the site of implantation. Polyester patches were implanted into the Wistar rat aorta or inferior vena cava and explanted on day 7 or 30. Neointima that formed on polyester patches was thicker in the venous environment compared to the amount that formed on patches in the arterial environment. Venous patches had more cell proliferation and greater numbers of VCAM-positive and CD68-positive cells, whereas arterial patches had greater numbers of vimentin-positive and alpha-actin-positive cells. Although there were similar numbers of endothelial progenitor cells in the neointimal endothelium, cells in the arterial patch were Ephrin-B2- and notch-4-positive while those in the venous patch were Eph-B4- and COUP-TFII-positive. Venous patches treated with an arteriovenous fistula had decreased neointimal thickness; neointimal endothelial cells expressed Ephrin-B2 and notch-4 in addition to Eph-B4 and COUP-TFII. Polyester patches in the venous environment acquire venous identity, whereas patches in the arterial environment acquire arterial identity; patches in the fistula environment acquire dual arterial-venous identity. These data suggest that synthetic patches heal by acquisition of identity of their environment.

KEYWORDS:

arteriovenous fistula; endothelial cell identity; neointimal hyperplasia; patch angioplasty; polyester patch

PMID:
28877393
PMCID:
PMC5918420
DOI:
10.1002/jbm.a.36193
[Indexed for MEDLINE]
Free PMC Article

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