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Food Res Int. 2017 Oct;100(Pt 1):873-884. doi: 10.1016/j.foodres.2017.06.052. Epub 2017 Jul 2.

The novel contributors of anti-diabetic potential in mulberry polyphenols revealed by UHPLC-HR-ESI-TOF-MS/MS.

Author information

1
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
2
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: chengu@scut.edu.cn.
3
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address: lfxfu@scut.edu.cn.

Abstract

To explore the comprehensive phenolic profile of mulberry (Morus alba L.) fruit and reveal the novel anti-diabetic constituents, mulberry polyphenols (MP) were fractionated through gradient ethanol elution and subjected to composition and bioactivity analysis. Forty-seven phenolic compounds were identified and quantified through UHPLC-HR-ESI-TOF-MS/MS, including twenty-one newly identified compounds such as delphinidin 3-rutinoside-5-glucoside and cyanidin 3-rutinoside dimer. The antioxidant activity and anti-diabetic potential of fractionated MP were analyzed in vitro and ex vivo with HepG2 and pancreatic β-cell RIN-m5F. Hierarchical cluster analysis and Pearson correlation coefficients revealed compounds such as syringic acid and galloylcyanidin-glycoside contributed most to inhibit α-glucosidase activity. Quercetin and cyanidin-glycosides were essential for cellular antioxidant activity. Dihydroquercetin and 1,5-dicaffeoylquinic acid were newly identified and validated as the main contributors after rutin to protect RIN-m5F cells against glucotoxicity. Such findings suggest the promising role of MP for type II diabetics and lay the foundation of further utilization and investigation.

KEYWORDS:

1,5-Dicaffeoylquinic acid (PubChem CID: 122,685); Caffeic acid (PubChem CID: 689043); Correlation analysis; Cyanidin 3-glucoside (PubChem CID: 44256715); Cyanidin 3-rutinoside (PubChem CID: 441674); Cyanidin 3-sophoroside (PubChem CID: 44256720); Hierarchical cluster analysis; Kaempferol-3-rutinoside (PubChem CID: 5318767); Mulberry polyphenols; Pancreatic β-cell RIN-m5F; Quercetin (PubChem CID: 5280343); Rutin (PubChem CID: 5280805); Sinapic acid (PubChem CID: 637775); UHPLC-HR-ESI-TOF-MS/MS

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