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Food Chem. 2018 Jan 15;239:640-648. doi: 10.1016/j.foodchem.2017.06.138. Epub 2017 Jun 27.

Methodological evaluation of DNA-based molecular keys to identify categories of mislabelling in commercial products from genus Merluccius spp.

Author information

1
Instituto Español de Oceanografía, Centro Oceanográfico de Vigo, 36200 Vigo, Spain. Electronic address: montse.perez@ieo.es.
2
University of Vigo, Faculty of Biology, Department of Biochemistry, Genetics and Immunology, 36310 Vigo, Spain. Electronic address: angie.santafe@hotmail.com.
3
University of Vigo, Faculty of Biology, Department of Biochemistry, Genetics and Immunology, 36310 Vigo, Spain. Electronic address: miguel.balado@usc.es.
4
University of Vigo, Faculty of Biology, Department of Biochemistry, Genetics and Immunology, 36310 Vigo, Spain. Electronic address: pressa@uvigo.es.

Abstract

The large variety of fish formats which are globally commercialized supports use of meta-evaluation studies to test discrimination power among molecular keys available for traceability of highly-degraded and/or chemically-modified DNA material. This paper shows that a combination of DNA identification methods validated for genus Merluccius allows 100% species assignment in hake products and offers higher diagnostic power (97% on products) than individual methods, i.e. Hake-ITS1-RFLP (89%) or Hake-Cytochrome b-RFLP (83%). A global 31% product mislabelling involved 15% of products affected by internal species substitution, as the main cause of mislabelling in ultrafrozen products, and another 16% affected by external species substitution with non-hake species, as the cause of mislabelling in processed and cooked hake-based products. The combination of both DNA keys minimizes the tool-associated rate of diagnostic failure, allowing decoupling of mislabelling categories and maximizing the quantitative adscription of products to species on any kind of hake-based products.

KEYWORDS:

Commercial mislabelling; Commercial traceability; Cytochrome b-RFLP; Hake ITS1-RFLP; Hakes; Merluccius spp.; Phylodiagnosis; Species authentication

PMID:
28873616
DOI:
10.1016/j.foodchem.2017.06.138
[Indexed for MEDLINE]

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