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Mycoses. 2018 Jan;61(1):48-52. doi: 10.1111/myc.12696. Epub 2017 Sep 26.

Candida-reactive T cells for the diagnosis of invasive Candida infection of the lumbar vertebral spine.

Author information

1
Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, Cologne, Germany.
2
Department I of Internal Medicine, University Hospital of Cologne, Cologne, Germany.
3
Clinical Trials Centre Cologne, ZKS Köln, Köln, Germany.
4
German Centre for Infection Research, Partner Site Bonn-Cologne, Cologne, Germany.
5
Labor Dr. Wisplinghoff, Cologne, Germany.
6
Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany.
7
Institute for Clinical Microbiology, University Witten/Herdecke, Witten, Germany.
8
Department of Diagnostic and Interventional Radiology, University Hospital of Cologne, Cologne, Germany.
9
Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.

Abstract

Invasive Candida infection is the fourth most common bloodstream infection. Blood cultures are the current gold standard diagnostic method, however, false negatives remain a clinical challenge. We developed a new technique measuring Candida-reactive T cells as diagnostic read-out for invasive Candida infection. In a pilot study, we followed the treatment course of a patient with an invasive Candida infection of the lumbar vertebral spine. We present the case of a 56-year-old patient with HIV-associated Burkitt lymphoma who developed septic shock during chemotherapy-induced neutropenia. For the first time, we provide flow cytometry-based diagnostics with Candida-reactive T cells for invasive candidiasis with comprehensive MRI imaging. The Candida-reactive T cell assay has potential to complement current diagnostic assays for invasive Candida infection and thus to support targeted treatment.

KEYWORDS:

CD154; Candida spondylodiscitis; candidaemia; flow cytometry; invasive candidiasis; osteomyelitis

PMID:
28872711
DOI:
10.1111/myc.12696
[Indexed for MEDLINE]

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