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J Clin Invest. 2017 Oct 2;127(10):3717-3729. doi: 10.1172/JCI94130. Epub 2017 Sep 5.

mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy.

Author information

1
Telethon Institute of Genetics and Medicine (TIGEM), and.
2
Dulbecco Telethon Institute, Pozzuoli, Naples, Italy.
3
Medical Genetics Unit, Department of Medical and Translational Science, Federico II University, Naples, Italy.
4
Department of Pediatrics, Dulbecco Telethon Institute at Centro Ricerca Tettamanti, University of Milano-Bicocca, Monza, Italy.
5
Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas, USA and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
6
Department of Molecular and Human Genetics, Baylor College of Medicine, and.
7
Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, Texas, USA.

Abstract

The mammalian target of rapamycin complex 1 (mTORC1) kinase promotes cell growth by activating biosynthetic pathways and suppressing catabolic pathways, particularly that of macroautophagy. A prerequisite for mTORC1 activation is its translocation to the lysosomal surface. Deregulation of mTORC1 has been associated with the pathogenesis of several diseases, but its role in skeletal disorders is largely unknown. Here, we show that enhanced mTORC1 signaling arrests bone growth in lysosomal storage disorders (LSDs). We found that lysosomal dysfunction induces a constitutive lysosomal association and consequent activation of mTORC1 in chondrocytes, the cells devoted to bone elongation. mTORC1 hyperphosphorylates the protein UV radiation resistance-associated gene (UVRAG), reducing the activity of the associated Beclin 1-Vps34 complex and thereby inhibiting phosphoinositide production. Limiting phosphoinositide production leads to a blockage of the autophagy flux in LSD chondrocytes. As a consequence, LSD chondrocytes fail to properly secrete collagens, the main components of the cartilage extracellular matrix. In mouse models of LSD, normalization of mTORC1 signaling or stimulation of the Beclin 1-Vps34-UVRAG complex rescued the autophagy flux, restored collagen levels in cartilage, and ameliorated the bone phenotype. Taken together, these data unveil a role for mTORC1 and autophagy in the pathogenesis of skeletal disorders and suggest potential therapeutic approaches for the treatment of LSDs.

PMID:
28872463
PMCID:
PMC5617676
DOI:
10.1172/JCI94130
[Indexed for MEDLINE]
Free PMC Article

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