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Sci Rep. 2017 Sep 4;7(1):10340. doi: 10.1038/s41598-017-10622-5.

Central Nervous System Responses of the Oriental migratory, Locusta migratoria manilensis, to Fungal Infection.

Zhang W1,2,3,4, Chen J1,2,3, Keyhani NO1,2,3,4, Jin K1,2,3, Wei Q1,2,3, Xia Y5,6,7.

Author information

1
Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, 400045, PR China.
2
Chongqing Engineering Research Center for Fungal Insecticide, Chongqing, 400045, PR China.
3
Key Laboratory of Gene Function and Regulation Technologies under Chongqing Municipal Education Commission, Chongqing, 400045, PR China.
4
Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA.
5
Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, 400045, PR China. yuxianxia@cqu.edu.cn.
6
Chongqing Engineering Research Center for Fungal Insecticide, Chongqing, 400045, PR China. yuxianxia@cqu.edu.cn.
7
Key Laboratory of Gene Function and Regulation Technologies under Chongqing Municipal Education Commission, Chongqing, 400045, PR China. yuxianxia@cqu.edu.cn.

Abstract

Responses of the central nervous system (CNS) to microbial challenge and the interplay between the CNS and the immune system are important for defending against pathogen attack. We have examined the CNS transcriptional response of Locusta migratoria manilensis to infection by the locust-specific fungal pathogen, Metarhizium acridum. CNS responses were examined during spore attachment, fungal germination and pre-penetration of the cuticle, and cuticle penetration/hemocoel ingress and proliferation. Effects were seen at the earliest time points (4 h post-infection) and the number of differentially expressed genes (DEGs) was highest during late mycosis (72 h post-infection). Significantly affected neurological pathways included genes involved in serotonergic, cholinergic, dopaminergic, GABAergic, and glutamergic synapse responses, as well as pathways responsible for synaptic vesicle cycle, long-term potentiation and depression, and neurotrophin and retrograde endocannabinoid signaling. In addition, a significant number of immune related DEGs were identified. These included components of the Toll, Imd and JAK/STAT pathways, consistent with interactions between the CNS and immune systems. The activation of immune response related CNS genes during early stage infection highlights the rapid detection of microbial pathogens and suggests an important role for the CNS in modulating immunity potentially via initiating behavioral adaptations along with innate immune responses.

PMID:
28871168
PMCID:
PMC5583336
DOI:
10.1038/s41598-017-10622-5
[Indexed for MEDLINE]
Free PMC Article

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