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Nature. 2017 Sep 14;549(7671):233-237. doi: 10.1038/nature23649. Epub 2017 Sep 6.

Structural basis of MsbA-mediated lipopolysaccharide transport.

Author information

1
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
2
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
3
Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, Maryland 21201, USA.
4
Laboratory of Molecular Electron Microscopy, The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA.

Abstract

Lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria is critical for the assembly of their cell envelopes. LPS synthesized in the cytoplasmic leaflet of the inner membrane is flipped to the periplasmic leaflet by MsbA, an ATP-binding cassette transporter. Despite substantial efforts, the structural mechanisms underlying MsbA-driven LPS flipping remain elusive. Here we use single-particle cryo-electron microscopy to elucidate the structures of lipid-nanodisc-embedded MsbA in three functional states. The 4.2 Å-resolution structure of the transmembrane domains of nucleotide-free MsbA reveals that LPS binds deep inside MsbA at the height of the periplasmic leaflet, establishing extensive hydrophilic and hydrophobic interactions with MsbA. Two sub-nanometre-resolution structures of MsbA with ADP-vanadate and ADP reveal an unprecedented closed and an inward-facing conformation, respectively. Our study uncovers the structural basis for LPS recognition, delineates the conformational transitions of MsbA to flip LPS, and paves the way for structural characterization of other lipid flippases.

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PMID:
28869968
PMCID:
PMC5759761
DOI:
10.1038/nature23649
[Indexed for MEDLINE]
Free PMC Article

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