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J Appl Microbiol. 2017 Nov;123(5):1148-1159. doi: 10.1111/jam.13578. Epub 2017 Oct 13.

Identification of α-tocopherol as a bioactive component of Dicranopteris linearis with disrupting property against preformed biofilm of Staphylococcus aureus.

Author information

1
School of Science, Monash University Malaysia, Bandar Sunway, Selangor, Malaysia.
2
Tropical Medicine and Biology Multidisciplinary Platform, Monash University Malaysia, Bandar Sunway, Selangor, Malaysia.
3
Structural and Biophysics Facility, Malaysia Genome Institute (MGI), National Institutes of Biotechnology Malaysia (NIBM), Kajang, Selangor, Malaysia.

Abstract

AIMS:

The potential of Dicranopteris linearis leaves' extract and its bioactive components were investigated for the first time for its disrupting ability against Staphylococcus aureus biofilms.

METHODS AND RESULTS:

The leaves of D. linearis were subjected to sonication-assisted extraction using hexane (HEX), dichloromethane, ethyl acetate and methanol (MeOH). It was found that only the MeOH fraction exhibited antimicrobial activity using broth microdilution assay; while all four fractions do not exhibit biofilm inhibition activity against S. aureusATCC 6538P, S. aureusATCC 43300, S. aureusATCC 33591 and S. aureusATCC 29213 using crystal violet assay. Among the four fractions tested, only the HEX fraction showed biofilm disrupting ability, with 60-90% disruption activity at 5 mg ml-1 against all four S. aureus strains tested. Bioassay-guided purification of the active fraction has led to the isolation of α-tocopherol. α-Tocopherol does not affect the cells within the biofilms but instead affects the biofilm matrix in order to disrupt S. aureus biofilms.

CONCLUSIONS:

α-Tocopherol was identified to be the bioactive component of D. linearis with disruption activity against S. aureus biofilm matrix.

SIGNIFICANCE AND IMPACT OF THE STUDY:

The use of α-tocopherol as a biofilm disruptive agent might potentially be useful to treat biofilm-associated infections in the future.

KEYWORDS:

Dicranopteris linearis ; Staphylococcus aureus ; biofilm; disruption; α-tocopherol

PMID:
28869803
DOI:
10.1111/jam.13578
[Indexed for MEDLINE]

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