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Nat Chem Biol. 2017 Nov;13(11):1158-1163. doi: 10.1038/nchembio.2457. Epub 2017 Sep 4.

An artificial transport metabolon facilitates improved substrate utilization in yeast.

Author information

1
Institute of Molecular Biosciences, Goethe University, Frankfurt am Main, Germany.
2
Molecular Bioenergetics, Center of Biological Chemistry, Cluster of Excellence Frankfurt Macromolecular Complexes, Goethe University, Frankfurt, Germany.
3
Functional Proteomics, Institute of Biochemistry I, Faculty of Medicine, Goethe University, Frankfurt, Germany.
4
Department of Biochemistry and Molecular Biology, Chicago Medical School, Rosalind Franklin University, North Chicago, Illinois, USA.

Abstract

Efficient substrate utilization is the first and most important prerequisite for economically viable production of biofuels and chemicals by microbial cell factories. However, production rates and yields are often compromised by low transport rates of substrates across biological membranes and their diversion to competing pathways. This is especially true when common chassis organisms are engineered to utilize nonphysiological feedstocks. Here, we addressed this problem by constructing an artificial complex between an endogenous sugar transporter and a heterologous xylose isomerase in Saccharomyces cerevisiae. Direct feeding of the enzyme through the transporter resulted in acceleration of xylose consumption and substantially diminished production of xylitol as an undesired side product, with a concomitant increase in the production of ethanol. This underlying principle could also likely be implemented in other biotechnological applications.

PMID:
28869594
DOI:
10.1038/nchembio.2457
[Indexed for MEDLINE]

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