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Microb Pathog. 2017 Oct;111:174-181. doi: 10.1016/j.micpath.2017.08.036. Epub 2017 Sep 1.

Borrelia burgdorferi basic membrane protein A could induce chemokine production in murine microglia cell line BV2.

Author information

1
Yunnan Province Key Laboratory for Tropical Infectious Diseases in Universities, Kunming 650500, China; Department of Microbiology and Immunology, Kunming Medical University, Kunming 650500, China.
2
Yunnan Province Key Laboratory for Tropical Infectious Diseases in Universities, Kunming 650500, China; The Institute for Tropical Medicine, Kunming Medical University, Kunming 650500, China; Yunnan Province Integrative Innovation Center for Public Health, Diseases Prevention and Control, Kunming Medical University, Kunming 650500, China; Yunnan Demonstration Base of International Science and Technology Cooperation for Tropical Diseases, Kunming 650500, China; Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming 650500, China. Electronic address: liuaihua@kmmu.edu.cn.
3
Yunnan Province Key Laboratory for Tropical Infectious Diseases in Universities, Kunming 650500, China; The Institute for Tropical Medicine, Kunming Medical University, Kunming 650500, China; Yunnan Province Integrative Innovation Center for Public Health, Diseases Prevention and Control, Kunming Medical University, Kunming 650500, China.
4
Yunnan Province Key Laboratory for Tropical Infectious Diseases in Universities, Kunming 650500, China; Department of Microbiology and Immunology, Kunming Medical University, Kunming 650500, China; The Institute for Tropical Medicine, Kunming Medical University, Kunming 650500, China; Yunnan Province Integrative Innovation Center for Public Health, Diseases Prevention and Control, Kunming Medical University, Kunming 650500, China; Yunnan Demonstration Base of International Science and Technology Cooperation for Tropical Diseases, Kunming 650500, China. Electronic address: baofukai@kmmu.edu.cn.

Abstract

Lyme neuroborreliosis is a nervous system infectious disease caused by Borrelia burgdorferi (B. burgdorferi). It has been demonstrated that cytokines induced by B. burgdorferi are related to Lyme neuroborreliosis. Microglia is known as a key player in the immune responses that occur within the central nervous system. In response to inflammation, it will be activated and generate cytokines and chemokines. Experiments in vitro cells have showed that B. Burgdorferi membrane protein A (BmpA), a major immunogen of B. Burgdorferi, could induce Lyme arthritis and stimulate human and murine lymphocytes to produce inflammatory cytokines. In our study, the murine microglia BV2 cell line was used as a cell model to explore the stimulating effects of recombinant BmpA (rBmpA); Chemokine chip, ELISA and QPCR technology were used to measure the production of chemokines from microglial cells stimulated by rBmpA. Compared with the negative control group, CXCL2, CCL22, and CCL5 concentrations in the cell supernatant increased significantly after the rBmpA stimulation; the concentration of these chemokines increased with rBmpA concentration increasing; the mRNA expression levels of chemokines (CXCL2, CCL22, and CCL5) in murine BV2 cells increased significantly with 10 μg/mL and 20 μg/mL rBmpA stimulation; CXCL13 was not change after the rBmpA stimulation. Our study shows that chemokines, such as CXCL2, CCL22, and CCL5 were up-regulated by the rBmpA in the BV2 cells. The production of chemokines in Lyme neuroborreliosis may be mainly from microglia cells and the rBmpA may be closely related with the development of Lyme neuroborreliosis.

KEYWORDS:

Borrelia burgdorferi; Chemokine; Lyme neuroborreliosis; Microglia cell; rBmpA

PMID:
28867633
DOI:
10.1016/j.micpath.2017.08.036
[Indexed for MEDLINE]

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