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J Inorg Biochem. 2018 Apr;181:177-182. doi: 10.1016/j.jinorgbio.2017.07.032. Epub 2017 Jul 29.

Relationship between aluminum stress and caffeine biosynthesis in suspension cells of Coffea arabica L.

Author information

1
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No. 130 X 32 y 34, Col. Chuburná de Hidalgo, 97205 Mérida, Yucatán, Mexico.
2
CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán, Periférico Norte, Km 33.5, Tablaje catastral 13615 Col. Chuburná de Hidalgo, 97203 Mérida, YUC, Mexico.
3
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No. 130 X 32 y 34, Col. Chuburná de Hidalgo, 97205 Mérida, Yucatán, Mexico. Electronic address: ths@cicy.mx.

Abstract

Toxicity by aluminum is a growth-limiting factor in plants cultivated in acidic soils. This metal also promotes signal transduction pathways leading to the biosynthesis of defense compounds, including secondary metabolites. In this study, we observed that Coffea arabica L. cells that were kept in the dark did not produce detectable levels of caffeine. However, irradiation with light and supplementation of the culture medium with theobromine were the best conditions for cell maintenance to investigate the role of aluminum in caffeine biosynthesis. The addition of theobromine to the cells did not cause any changes to cell growth and was useful for the bioconversion of theobromine to caffeine. During a short-term AlCl3-treatment (500μM) of C. arabica cells kept under light irradiation, increases in the caffeine levels in samples that were recovered from both the cells and culture media were evident. This augmentation coincided with increases in the enzyme activity of caffeine synthase (CS) and the transcript level of the gene encoding this enzyme (CS). Together, these results suggest that actions by Al and theobromine on the same pathway lead to the induction of caffeine biosynthesis.

KEYWORDS:

Aluminum; Caffeine; Caffeine synthase; Coffea arabica; Theobromine

PMID:
28867596
DOI:
10.1016/j.jinorgbio.2017.07.032
[Indexed for MEDLINE]

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