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Methods Mol Biol. 2017;1662:137-150. doi: 10.1007/978-1-4939-7262-3_12.

Analysis of Phragmoplast Kinetics During Plant Cytokinesis.

Author information

1
Developmental Genetics, Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany.
2
Cellular Nanoscience, Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany.
3
Developmental Genetics, Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany. sabine.mueller@zmbp.uni-tuebingen.de.

Abstract

In plants, the partitioning of daughter cells during cytokinesis is achieved via physical insertion of a membranous cell plate within the dividing parent cell. It is a cellular process of extensive protein secretion and membrane trafficking toward the plane of cell division and the cytoskeleton is an important facilitator of this process. A specialized cytoskeletal array termed phragmoplast expands centrifugally throughout cytokinesis and directs, mostly Golgi-derived vesicles that ultimately fuse to form the developing cell plate. The function of the phragmoplast in guiding cell plate synthesis has strongly motivated many scientists to monitor its dynamic behavior. In this chapter, we present an overview of basic principles and methods concerning the live imaging of cytokinetic plant cells using confocal laser scanning microscopy (CLSM) and the analysis of phragmoplast expansion.

KEYWORDS:

Arabidopsis; CLSM; Cell plate; Cytokinesis; FM4-64; GFP; MAP4; MBD; Microtubules; Phragmoplast

PMID:
28861824
DOI:
10.1007/978-1-4939-7262-3_12
[Indexed for MEDLINE]

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