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Am J Physiol Lung Cell Mol Physiol. 2018 Jan 1;314(1):L107-L117. doi: 10.1152/ajplung.00277.2017. Epub 2017 Aug 31.

The respiratory tract microbial biogeography in alcohol use disorder.

Author information

1
Department of Medicine, Section of Pulmonary/Critical Care & Allergy/Immunology, Louisiana State University Health Sciences Center , New Orleans, Louisiana.
2
Department of Medicine, Division of Pulmonary Sciences & Critical Care, University of Colorado-Anschutz Medical Campus , Aurora, Colorado.
3
Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center , New Orleans, Louisiana.

Abstract

Individuals with alcohol use disorders (AUDs) are at an increased risk of pneumonia and acute respiratory distress syndrome. Data of the lung microbiome in the setting of AUDs are lacking. The objective of this study was to determine the microbial biogeography of the upper and lower respiratory tract in individuals with AUDs compared with non-AUD subjects. Gargle, protected bronchial brush, and bronchoalveolar lavage specimens were collected during research bronchoscopies. Bacterial 16S gene sequencing and phylogenetic analysis was performed, and the alterations to the respiratory tract microbiota and changes in microbial biogeography were determined. The microbial structure of the upper and lower respiratory tract was significantly altered in subjects with AUDs compared with controls. Subjects with AUD have greater microbial diversity [ P < 0.0001, effect size = 16 ± 1.7 observed taxa] and changes in microbial species relative abundances. Furthermore, microbial communities in the upper and lower respiratory tract displayed greater similarity in subjects with AUDs. Alcohol use is associated with an altered composition of the respiratory tract microbiota. Subjects with AUDs demonstrate convergence of the microbial phylogeny and taxonomic communities between distinct biogeographical sites within the respiratory tract. These results support a mechanistic pathway potentially explaining the increased incidence of pneumonia and lung diseases in patients with AUDs.

KEYWORDS:

alcohol; biogeography; lung; microbiome; respiratory

PMID:
28860145
PMCID:
PMC5866426
DOI:
10.1152/ajplung.00277.2017
[Indexed for MEDLINE]
Free PMC Article

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