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Biosci Biotechnol Biochem. 2017 Oct;81(10):1899-1907. doi: 10.1080/09168451.2017.1364965. Epub 2017 Sep 1.

MicroRNA-34a induces transdifferentiation of glioma stem cells into vascular endothelial cells by targeting Notch pathway.

Author information

1
a Key Laboratory of Cancer Prevention and Treatment of Heilongjiang Province , Mudanjiang Medical University , Mudanjiang , People's Republic of China.
2
b Department of Pathology , Mudanjiang Medical University , Mudanjiang , People's Republic of China.
3
c Department of Rehabilitation Medicine , Hongqi Hospital of Mudanjiang Medical University , Mudanjiang , People's Republic of China.
4
d Department of Cardiology , Hongqi Hospital of Mudanjiang Medical University , Mudanjiang , People's Republic of China.
5
e Department of Scientific Research , Mudanjiang Medical University , Mudanjiang , People's Republic of China.
6
f Department of Thoracic Surgery , Hongqi Hospital of Mudanjiang Medical University , Mudanjiang , People's Republic of China.

Abstract

The function of microRNA-34a (miR-34a) in transdifferentiation of glioma stem cells (GSCs) into vascular endothelial cells (VECs) was explored by focusing on Notch ligand Delta-like 1 (Dll1). MiR-34a mimics was transfected into CD133 + glioma cell U251. The angiogenesis feature of miR-34a transfected U251 cells was investigated and the expressions of CD31, CD34, Vwf, Notch 1, and Dll1 were quantified. Length of branching vessel-like structures in the miR-34a transfected U251 cells was significantly higher than control cells. The VEC feature of miR-34a overexpressed U251 cells was further confirmed by the expressions of CD31, CD34, and vWF. Transfection of miR-34a decreased the expression of Notch 1 and Dll1. Furthermore, the miR-34a overexpression-enhanced tube formation of GSCs was suppressed when the decreased expression of Dll1 was restored. The current study highlighted the potential of miR-34a as an inducer in GSCs' transdifferentiation into VECs by targeting Dll1.

KEYWORDS:

Notch 1; delta like 1; glioma stem cells; microRNA 34a; vascular endothelial cells

PMID:
28859546
DOI:
10.1080/09168451.2017.1364965
[Indexed for MEDLINE]

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