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Int J Mol Sci. 2017 Aug 31;18(9). pii: E1879. doi: 10.3390/ijms18091879.

Health-Promoting Effects of Thymus herba-barona, Thymus pseudolanuginosus, and Thymus caespititius Decoctions.

Author information

1
Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal. andrea.afonso@ulsne.min-saude.pt.
2
Public Health Laboratory of Bragança, Local Health Unit, Rua Eng. Adelino Amaro da Costa, 5300-146 Bragança, Portugal. andrea.afonso@ulsne.min-saude.pt.
3
Department of Diagnostic and Therapeutic Technologies, School of Health Sciences, Polytechnic Institute of Bragança, Av. D. Afonso V, 5300-121 Bragança, Portugal. oliviapereira@ipb.pt.
4
Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal. rodrigotrepaneto@gmail.com.
5
Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal. artur.silva@ua.pt.
6
Department of Chemistry & QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal. susanacardoso@ua.pt.

Abstract

Thymus herba-barona, Thymus pseudolanuginosus, and Thymus caespititius decoctions were screened for their phenolic constituents, along with their potential antioxidant, anti-inflammatory, and antibacterial activities. The total phenolic compounds in the extracts of the three plants ranged from 236.0 ± 26.6 mgGAE/g (T. caespititus) to 293.0 ± 30.5 mgGAE/g of extract (T. pseudolanuginosus), being particularly rich in caffeic acid derivatives, namely rosmarinic acid and its structural isomers, as well as flavones, such as luteolin-O-glucuronide. The T. pseudolanuginosus extract presented the best DPPH radical scavenging ability (EC50 = 10.9 ± 0.7 µg/mL), a high reducing power (EC50 = 32.2 ± 8.2 µg/mL), and effectively inhibited the oxidation of β-carotene (EC50 = 2.4 ± 0.2 µg/mL). The extracts also showed NO scavenging activity close to that of ascorbic acid, and thus might be useful as anti-inflammatory agents. In addition, they exhibited antibacterial activity against gram-negative and gram-positive bacteria. Staphylococcus aureus strains were the most sensitive bacteria to thyme extracts, with minimum inhibitory concentration and minimum bactericidal concentration values in the range of 0.6-3.5 mg/mL. Overall, this work is an important contribution for the phytochemical characterization and the potential antioxidant, anti-inflammatory, and antimicrobial activities of these three Thymus species, which have been poorly explored.

KEYWORDS:

LC-MS; anti-inflammatory; antimicrobial activity; antioxidant; antiradicalar; mass spectrometry; phenolic; thyme; thymus

PMID:
28858228
PMCID:
PMC5618528
DOI:
10.3390/ijms18091879
[Indexed for MEDLINE]
Free PMC Article

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