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Tumour Biol. 2017 Aug;39(8):1010428317720940. doi: 10.1177/1010428317720940.

N-glycosylation of the transient receptor potential melastatin 8 channel is altered in pancreatic cancer cells.

Author information

1
1 Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, Bucharest, Romania.
2
2 Department of Molecular Cell Biology, Institute of Biochemistry, Romanian Academy, Bucharest, Romania.

Abstract

Transient receptor potential melastatin 8 (TRPM8), a membrane ion channel, is activated by thermal and chemical stimuli. In pancreatic ductal adenocarcinoma, TRPM8 is required for cell migration, proliferation, and senescence and is associated with tumor size and pancreatic ductal adenocarcinoma stages. Although the underlying mechanisms of these processes have yet to be described, this cation-permeable channel has been proposed as an oncological target. In this study, the glycosylation status of the TRPM8 channel was shown to affect cell proliferation, cell migration, and calcium uptake. TRPM8 expressed in the membrane of the Panc-1 pancreatic tumoral cell line is non-glycosylated, whereas human embryonic kidney cells transfected with human TRPM8 overexpress a glycosylated protein. Moreover, our data suggest that Ca2+ uptake is modulated by the glycosylation status of the protein, thus affecting cell proliferation.

KEYWORDS:

Pancreatic cancer; cell migration; glycosylation; membrane protein; patch clamp; transient receptor potential melastatin 8

PMID:
28857015
DOI:
10.1177/1010428317720940
[Indexed for MEDLINE]

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