Format

Send to

Choose Destination
Genes Nutr. 2017 Aug 24;12:20. doi: 10.1186/s12263-017-0573-3. eCollection 2017.

Lactase persistence in Tunisia as a result of admixture with other Mediterranean populations.

Author information

1
Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, BP 74, 13 Place Pasteur, 1002 Tunis, Tunisia.
2
Université de Tunis El Manar, 2092 El Manar I, Tunis, Tunisia.
3
Laboratory of Molecular Anthropology and Centre for Genome Biology, Department of Biological, Geological and Environmental Sciences (BiGeA), University of Bologna, 40126 Bologna, Italy.
4
Department of external consultation, National Institute of Nutrition and Food Technology, Tunis, Tunisia.
5
Laboratory of Genetic Medicine and Immunology, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha, Qatar.
6
Medical Genetics Unit, Department of Medical and Surgical Sciences, Polyclinic S. Orsola-Malpighi, University of Bologna, Bologna, Italy.

Abstract

BACKGROUND:

The ability to digest lactose after weaning, namely, lactase persistence (LP), is encoded by polymorphisms in the MCM6 gene and varies widely in frequency among different human populations. Although, evolution of LP-related genetic variants was investigated in many groups of Sub-Saharan African, Middle Eastern, and European ancestry, only few studies have focused on populations from North Africa and no data are especially available from the Tunisian one. For this reason, there is an urgent need to investigate the frequency patterns at these loci in Tunisia since this adaptive trait is implicated in health.

METHODS:

Forty SNPs covering the LCT/MCM6 genes and including the two functional variants - 13,910 C > T and - 22,018 G > A were genotyped in 117 Tunisian individuals using the Sequenom Mass Array technology. The observed nucleotide and haplotype patterns of variation were then compared with those of several African, European, and Mediterranean human groups for which comparable data were publicly available. Admixture analysis on a 5 Mb genomic region surrounding the LCT/MCM6 loci was also performed by extracting genotypes from a previously generated genome-wide dataset in order to deepen the reconstruction of the evolutionary history of these loci.

RESULTS:

We found that lactase non-persistence (LNP)-related alleles and haplotypes were predominantly present in the examined population. A clear differentiation between Tunisian, African, and North European/North Italian samples was found, while the Tunisian population showed more genetic affinity to Central and South Italian groups.

CONCLUSIONS:

Our study provided a first report of LP-associated alleles and haplotypes in the Tunisian population. We highlighted a gradient followed by LP diffusion from Europe to North Africa. Based on the rich historic background of Tunisia, we suggest that this adaptive trait was introduced in that geographic region by a relatively recent gene flow.

KEYWORDS:

Admixture; LCT; Lactase persistence; MCM6; Natural selection; North Africa; Tunisia

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center