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FASEB J. 2017 Dec;31(12):5478-5494. doi: 10.1096/fj.201700531R. Epub 2017 Aug 30.

Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise.

Author information

1
Mary MacKillop Institute for Health Research, Centre for Exercise and Nutrition, Australian Catholic University, Victoria, Australia.
2
Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, United Kingdom.
3
Department of Applied Mathematics, Liverpool John Moores University, Liverpool, United Kingdom.
4
Mary MacKillop Institute for Health Research, Centre for Exercise and Nutrition, Australian Catholic University, Victoria, Australia; john.hawley@acu.edu.au.

Abstract

It is generally accepted that muscle adaptation to resistance exercise (REX) training is underpinned by contraction-induced, increased rates of protein synthesis and dietary protein availability. By using dynamic proteome profiling (DPP), we investigated the contribution of both synthesis and breakdown to changes in abundance on a protein-by-protein basis in human skeletal muscle. Age-matched, overweight males consumed 9 d of a high-fat, low-carbohydrate diet during which time they either undertook 3 sessions of REX or performed no exercise. Precursor enrichment and the rate of incorporation of deuterium oxide into newly synthesized muscle proteins were determined by mass spectrometry. Ninety proteins were included in the DPP, with 28 proteins exhibiting significant responses to REX. The most common pattern of response was an increase in turnover, followed by an increase in abundance with no detectable increase in protein synthesis. Here, we provide novel evidence that demonstrates that the contribution of synthesis and breakdown to changes in protein abundance induced by REX differ on a protein-by-protein basis. We also highlight the importance of the degradation of individual muscle proteins after exercise in human skeletal muscle.-Camera, D. M., Burniston, J. G., Pogson, M. A., Smiles, W. J., Hawley, J. A. Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise.

KEYWORDS:

abundance; adaptation; metabolism; muscle protein synthesis; protein degradation

PMID:
28855275
DOI:
10.1096/fj.201700531R
[Indexed for MEDLINE]

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