Format

Send to

Choose Destination
Gigascience. 2017 Aug 1;6(8):1-7. doi: 10.1093/gigascience/gix055.

Large-scale phenomics analysis of a T-DNA tagged mutant population.

Wu HP1, Wei FJ1, Wu CC1,2, Lo SF3,4, Chen LJ5, Fan MJ6, Chen S7, Wen IC7, Yu SM3,4,8, Ho TD1,4,8, Lai MH9, Hsing YC1,10.

Author information

1
Institute of Plant and Microbial Biology, Academia Sinica, 128, Section 2, Yien-chu-yuan Road, Nankang, Taipei 115, Taiwan.
2
Institute of Plant Biology, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
3
Institute of Molecular Biology, Academia Sinica, 128, Section 2, Yien-chu-yuan Road, Nankang, Taipei 115, Taiwan.
4
Agricultural Biotechnology Center, National Chung Hsing University, 145, Xingda Road, Taichung 402, Taiwan.
5
Institute of Molecular Biology, National Chung Hsing University, 145, Xingda Road, Taichung 402, Taiwan.
6
Department of Biotechnology, Asia University, 500, Lioufeng Road, Taichung 413, Taiwan.
7
Plant Germplasm Division, Taiwan Agricultural Research Institute, 189, Zhongzheng Road, Taichung 413, Taiwan.
8
Department of Life Sciences, National Chung Hsing University, 145, Xingda Road, Taichung 402, Taiwan.
9
Crop Science Division, Taiwan Agricultural Research Institute, 189, Zhongzheng Road, Taichung 413, Taiwan.
10
Department of Agronomy, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.

Abstract

Rice, Oryza sativa L., is one of the most important crops in the world. With the rising world population, feeding people in a more sustainable and environmentally friendly way becomes increasingly important. Therefore, the rice research community needs to share resources to better understand the functions of rice genes that are the foundation for future agricultural biotechnology development, and one way to achieve this goal is via the extensive study of insertional mutants. We have constructed a large rice insertional mutant population in a japonica rice variety, Tainung 67. The collection contains about 93 000 mutant lines, among them 85% with phenomics data and 65% with flanking sequence data. We screened the phenotypes of 12 individual plants for each line grown under field conditions according to 68 subcategories and 3 quantitative traits. Both phenotypes and integration sites are searchable in the Taiwan Rice Insertional Mutants Database. Detailed analyses of phenomics data, T-DNA flanking sequences, and whole-genome sequencing data for rice insertional mutants can lead to the discovery of novel genes. In addition, studies of mutant phenotypes can reveal relationships among varieties, cultivation locations, and cropping seasons.

KEYWORDS:

T-DNA insertional mutants; flanking sequence; large-scale phenomics; rice; sequence analysis

PMID:
28854617
PMCID:
PMC5570018
DOI:
10.1093/gigascience/gix055
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center