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Nat Biotechnol. 2017 Oct;35(10):936-939. doi: 10.1038/nbt.3973. Epub 2017 Aug 30.

Multiplexed quantification of proteins and transcripts in single cells.

Author information

1
Genetics &Pharmacogenomics, Department of Translational Medicine, Merck &Co., Inc., Boston, Massachusetts, USA.
2
Informatics IT, Merck &Co., Inc., Boston, Massachusetts, USA.
3
Department of Profiling and Expression, Merck &Co., Inc., Palo Alto, California, USA.
4
Protein Sciences, Department of Biologics, Merck &Co., Inc., Boston, Massachusetts, USA.

Abstract

We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type.

PMID:
28854175
DOI:
10.1038/nbt.3973
[Indexed for MEDLINE]

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